Articular cartilage restoration and regeneration stent and preparation method thereof

Abstract

The invention discloses an articular cartilage restoration and regeneration stent and a preparation method thereof, belonging to the field of medical materials. The stent is made of decalcified spongiosa-cortical bone, and the decalcified spongiosa-cortical bone is provided with more than two holes penetrating through the decalcified spongiosa bone and the cortical bone. The holes are vertically arranged on the horizontal surface of the decalcified cortical bone and have diameter of 0.1-1mm. The width between hole centers is 0.5-5mm. The preparation method provided by the invention comprises the steps of: soaking the bone in a decalcified solution for decalcification; monitoring the decalcification process by using an atomic absorption spectrophotometer; carrying out X-ray and CT detection on a decalcified sample to prove that the bone is completely decalcified to obtain the decalcified spongiosa-cortical bone; drilling on the horizontal surface of the decalcified cortical bone by using laser in a diameter of 0.1-1mm; and cutting the decalcified cortical bone into a certain size, and freezing and storing. The articular cartilage restoration and regeneration stent is made of a natural collagen stent material without immunological rejection reaction, is easy to adsorb by cells, and has good biocompatibility and better mechanical strength.

Description

technical field [0001] The invention relates to the field of medical materials, in particular to a scaffold for articular cartilage repair and regeneration and a preparation method thereof. Background technique [0002] Articular cartilage injury is an intractable injury in the field of sports medicine. The incidence of articular cartilage injury caused by exercise is very high. According to the survey results of the Institute of Sports Medicine of Peking University, cartilage injury accounts for more than 40.5% of knee joint sports injuries. Cartilage damage often causes symptoms such as joint swelling and pain. Due to the special structure of cartilage tissue, there are no nerves, blood vessels and lymphatic vessels in it, and the nutrient supply of cartilage is through the pressure changes generated by joint activities, so that the synovial fluid communicates between the joint cavity and the cartilage matrix, so the damaged articular cartilage cannot Repair and regenera...

AI Technical Summary

Problems solved by technology

[0004] In the process of realizing the present invention, the inventors found that the prior art has at least the following problems: the material properties of natural scaffold materials cannot be changed, and the mechanical strength and degradation rate are difficult to control, etc.

Commonly used natural scaffold materials include collagen, hydroxyapatite, etc. The biomechanical properties of natural scaffolds are difficult to adjust, and it is difficult to find biomechanical properties similar to those of cartilage.

Some natural biomaterial scaffolds have poor mechanical mechanics and cannot resist the mechanical stress generated during joint movement, which may easily cause the repair site to collapse and also affect the growth of cells on the material. For example, the elasticity and creep of hydroxyapatite scaffolds are higher than those of cartilage. Poor, too high hardness can easily cause wear of the corresponding cartilage articular surface

The biocompatibility of artificial scaffold materials is relatively poor, and there are different degrees of inflammatory reactions, as well as immunogenicity problems, and even carcinogenicity problems

Images