Process for decalcifying and extracting collagen from turtle carapace by utilizing ultrasonic assistance

Abstract

The invention discloses a process for decalcifying and extracting collagen from turtle carapace by utilizing ultrasonic assistance, wherein the process comprises the steps: mixing a clean turtle carapace sample with hydrochloric acid according to a solid-liquid ratio of 1:(10-50), and decalcifying for 15-120 minutes in an environment with the temperature of 15-38 DEG C and the ultrasonic power of50-250 W; and after the decalcification is finished, centrifugally collecting supernatant, and freeze-drying to obtain the carapace collagen. The research on the physicochemical properties of the obtained carapace collagen proves that the collagen migration rate of the carapace collagen extracted by the process is low, the decalcification rate is high, a basis is provided for the research on the further extraction of the collagen from the carapace of the Chinese soft shell turtle, and an important scientific basis is provided for aquatic product processing by-products and comprehensive utilization thereof.

Description

technical field [0001] The invention relates to the technical field of collagen extraction, in particular to a process for decalcifying and extracting collagen from soft-shelled turtle carapace with the aid of ultrasound. Background technique [0002] The main components of aquatic animal bones are collagen, connective tissue and minerals. Animal bones (such as bovine bones, etc.) usually contain rich proteins, polypeptides, amino acids, calcium, phosphorus and other minerals, and the bone protein is mainly collagen. It accounts for about 80% of the total protein; bone collagen is different from skin collagen, because bones contain about 60% to 70% of inorganic minerals, calcium phosphate and hydroxyapatite combine with collagen to form hard and insoluble bone Salt is deposited on the surface of collagen, so decalcification should be carried out before hydrolyzing collagen to reduce the content of inorganic impurities in collagen and obtain high-purity collagen. serious was...

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Problems solved by technology

[0002] The main components of aquatic animal bones are collagen, connective tissue and minerals. Animal bones (such as bovine bones) are usually rich in protein, peptides, amino acids and calcium , phosphorus and other minerals, and bone protein is mainly collagen, accounting for about 80% of the total protein; bone collagen is different from skin collagen, because bones contain about 60% to 70% of inorganic minerals, calcium phosphate Combined with hydroxyapatite and collagen to form hard and insoluble bone salt deposited on the surface of collagen, so decalcification should be carried out before hydrolyzing collagen to reduce the content of inorganic impurities in collagen and obtain high-purity collagen, like the back of a soft-shelled turtle. Most of the first-class by-products cannot be used effectively and with high value, resulting in serious waste of resources and environmental pollution; therefore, it is particularly important to find an efficient and reasonable comprehensive utilization method for the by-products of aquatic product processing;

[0003] Scholars at home and abroad have done some research on the extraction and removal of soluble calcium in fish bones and fish scales, but there are problems such as low decalcification efficiency and long time; Hao Shuxian et al. Using tilapia skin as raw material, fish skin collagen was extracted by different methods, among which the collagen extracted by ultrasonic pretreatment for 6.5 hours and the collagen extracted by acid method were different in terms of electrical conductivity, amino acid composition, gel strength and intrinsic viscosity, and thermal denaturation temperature. The difference is very small, which indirectly shows that ultrasonic pretreatment will not change the primary structure of collagen; in addition, Li Defu et al. also confirmed this point by taking collagen solution as the research object. The study found that collagen was found after ultrasonic pretreatment. The triple helix structure formed by self-assembly will become looser under ultrasound and will not disappear or dissociate; Wang Meiying et al. Under the condition that the ratio of liquid to material is 15 times, the decalcification rate reaches 92.43%. However, there is no relevant report on the extraction of soluble calcium from the carapace of Chinese soft-shelled turtle; the loss of collagen increases with the longer decalcification time;

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