CH4 molecular marker denaturing gel gradient electrophoresis (DGGE) identifying method for aquaculture of catfish hybrids

Abstract

The invention relates to a CH4 molecular marker DGGE identifying method for aquaculture of catfish hybrids. Southern catfishes, catfishes and hybrids of the southern catfishes and the catfishes serve as objects of study, CH4 gene sections in catfishes are selected to serve as molecular markers, the sequence difference the CH4 gene sections between the southern catfishes and the catfishes, denaturing gel gradient electrophoresis detection is performed and the southern catfishes, the catfishes and hybrids of the southern catfishes and the catfishes are identified successfully. The method has the advantages of being fast in experiment, convenient to operate, visual in identification and free from sequencing.

Description

technical field [0001] The invention relates to a molecular marker identification method for fish hybrids, in particular to the CH of aquaculture catfish hybrids 4 Molecular marker DGGE identification method. Background technique [0002] Siluriformes are carnivorous demersal fishes and are one of the most developed teleosts in fish evolution. Southern catfish (also known as big mouth catfish) ( Silurus meridionalis Chen) and catfish ( Silurus asotus Linnaeus) belong to Siluriformes, Siluridae, and Silurus genus, both of which are delicious and have high nutritional value, and are high-value-added economic fish mainly cultured in various parts of my country. Southern catfish is distributed in the Pearl River, Minjiang River, Xiangjiang River, Yangtze River and other water systems in my country. It is a large and ferocious carnivorous fish. It has the characteristics of fast growth, large body, and strong disease resistance. It likes to inhabit open water bodies, and The...

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Problems solved by technology

[0004] At present, RAPD, AFLP, and microsatellites are all suitable for germplasm identification at the population level. However, RAPD detection efficiency is low. Usually, only about ten pairs of random primers can be used after trying dozens of pairs of random primers, and the reproducibility is not good; AFLP has poor reproducibility. High, can produce a large number (usually more than 100) of polymorphic bands, but it is not easy to operate, while amplifying inter-species differences, it exacerbates intra-species differences, and there are a large number of false positive results, which limits the accuracy of hybrid identification; microsatellites Although it has species specificity, codominance, high polymorphism and sensitivity, it has been widely used in hybrid identification and genetic introgression analysis, but the loci used to identify hybrids need to be species specific In the analysis process, it is necessary to judge the authenticity of the amplification products, especially when identifying the hybridization of related species, it is particularly important to correctly judge the size and number of alleles in related species, which greatly increases the microsatellite Difficulty of using

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