CH4 molecular marker denaturing gel gradient electrophoresis (DGGE) identifying method for aquaculture of catfish hybrids

Abstract

The invention relates to a CH4 molecular marker DGGE identifying method for aquaculture of catfish hybrids. Southern catfishes, catfishes and hybrids of the southern catfishes and the catfishes serve as objects of study, CH4 gene sections in catfishes are selected to serve as molecular markers, the sequence difference the CH4 gene sections between the southern catfishes and the catfishes, denaturing gel gradient electrophoresis detection is performed and the southern catfishes, the catfishes and hybrids of the southern catfishes and the catfishes are identified successfully. The method has the advantages of being fast in experiment, convenient to operate, visual in identification and free from sequencing.

Description

Technical field [0001] The present invention involves the molecular label appraisal method of fish hybrid, and the CH of the aquaculture catfish hybrid 4 Molecular labeling DGGE appraisal method. Background technique [0002] Symbly -shaped fish is a carnivorous underlying fish and is one of the most developed real fish in fish evolution.Southern 大 (also known as Daguchi) ( Silurus Meridionalis Chen) and 和 ( Silurus assotus Linnaeus belongs to the shape, the family, and the genus. The two have delicious taste and high nutritional value. They are high -value -added economy fish in major farming across my country.Southern 我 is distributed in the water system of the Pearl River, Fujian River, Xiangjiang River, and Yangtze River in my country. It is a large and fierce meat fish. It has the characteristics of fast growth, individual large, and strong disease resistance. The gravel river section of the bottom of the river and the bottom of the river has a large amount of eggs and a lon...

AI Technical Summary

Problems solved by technology

[0004] At present, RAPD, AFLP, and microsatellites are all suitable for germplasm identification at the population level. However, RAPD detection efficiency is low. Usually, only about ten pairs of random primers can be used after trying dozens of pairs of random primers, and the reproducibility is not good; AFLP has poor reproducibility. High, can produce a large number (usually more than 100) of polymorphic bands, but it is not easy to operate, while amplifying inter-species differences, it exacerbates intra-species differences, and there are a large number of false positive results, which limits the accuracy of hybrid identification; microsatellites Although it has species specificity, codominance, high polymorphism and sensitivity, it has been widely used in hybrid identification and genetic introgression analysis, but the loci used to identify hybrids need to be species specific In the analysis process, it is necessary to judge the authenticity of the amplification products, especially when identifying the hybridization of related species, it is particularly important to correctly judge the size and number of alleles in related species, which greatly increases the microsatellite Difficulty of using

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