Animal model replication method of chronic inflammation caused by nano-bacterial infection animal prostate

A technology of nano-bacteria and animal models, applied in the field of biomedicine

Inactive Publication Date: 2015-04-29
NO 371 HOSPITAL PLA
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Problems solved by technology

[0004] Regarding the possible etiological relationship between nanobacteria and type III prost

Method used

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  • Animal model replication method of chronic inflammation caused by nano-bacterial infection animal prostate

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Embodiment Construction

[0022] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0023] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0024] Such as figure 1 Shown, the present invention is achieved like this, a kind of nano-bacteria infection animal prostate causes the animal model replication method of chronic inflammation comprising:

[0025] S101. Cultivate the identified nanobacteria and collect the cultured bacteria, wash the collected bacteria with PBS solution with a pH of 7.2, dilute with normal saline, and prepare 3.0×10 8 cfu / ml nano-bacteria liquid suspension for experimental use...

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Abstract

The invention discloses an animal model replication method of chronic inflammation caused by nano-bacterial infection animal prostate. The animal model replication method comprises the steps of cultivating strains of identified nano-bacteria and collecting cultivated strains to prepare nano-bacterial solution suspension, grouping rats, manufacturing rat prostate infection models, collecting specimens, detecting cell factors in prostate tissues and collecting the nano-bacterial re-cultivated specimens. Experimental materials provided for animal experiments for researching the pathogenic effect of the nano-bacteria to the prostate by means of the animal model replication method have important significance on discovery of III-type prostatitis and calculous etiology and pathogenesis, relieving of patients' pain, reduction of fund expenditure for diseases and social medical organizations and saving of more medical resources.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method for replicating an animal model of chronic inflammation caused by nano-bacteria infecting animal prostate. Background technique [0002] According to the prostatitis classification method proposed by the International Prostatitis Cooperation Network in 1998, prostatitis is divided into four types. Type Ⅰ: acute bacterial prostatitis, refers to acute bacterial infection of the prostate; type Ⅱ: chronic bacterial prostatitis, refers to chronic recurrent bacterial infection of the prostate; type Ⅲ: chronic prostatitis / chronic pelvic pain syndrome (CP / CPPS), It is defined as a history of more than 3 months, pain or discomfort in the pelvic area, varying degrees of discomfort during urination or sexual intercourse, and this type is divided into two subtypes: inflammatory and non-inflammatory according to prostate massage fluid and white blood cell count in semen; Type ...

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Application Information

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IPC IPC(8): A61D7/00A61D1/00A61K35/74
CPCA61D7/00A61D1/00A61K35/74
Inventor 李军张新际吕宏迪郝少君王希东张正臣
Owner NO 371 HOSPITAL PLA
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