Tandem mass spectrometry method capable of simultaneously detecting five organic tin pesticide residues in livestock and poultry meat products

Abstract

The invention discloses a tandem mass spectrometry method capable of simultaneously detecting five organic tin pesticide residues in livestock and poultry meat products. The method comprises the following steps: determining the volume of to-be-added formic acid-acetonitrile according to the protein content in a sample, extracting, deriving and purifying organic tin; carrying out derived preparation on standard working liquid, wherein the five organic tins are azocyclotin, cyhexatin, triphenyltin hydroxide, triphenyltin chloride and fenbutatin oxide, and three derivatives are derived from the sample; obtaining a standard working curve by employing gas chromatography-triple quadrupole mass spectrometric detection; detecting the residual quantity from three organic tin pesticide derivatives by an external standard method; and carrying out quantitative analysis with a standard curve to obtain the concentration and the residual quantity of the sample organic tin pesticide. The GC-MS/MS detection method with extremely high sensitivity is utilized, so that the protein is determined to be a basis of the adding amount of extract liquid; primary and tandem mass spectrometry fracture paths for three organic tin derivatives are analyzed; a specific mass spectrometry condition is determined; and the tandem mass spectrometry method is a rapid and efficient method for detecting organic tin pesticides in animal food.

Description

technical field [0001] The invention belongs to the technical field of food detection, in particular to a secondary mass spectrometry method for simultaneously detecting five organotin pesticide residues in poultry and livestock meat products Background technique [0002] Tin triazolate, tricyclic tin, triphenyltin hydroxide, triphenyltin chloride and fenbutyltin are often used as acaricides and fungicides in agriculture, which have certain toxicity and have good environmental stability. Applied in pears, apples, citrus, tea trees, cotton, wheat, vegetables, flowers and other crops, the organotin pesticide residues in chicken and beef come from the feed containing organotin pesticide residues in the feeding process, and in poultry meat Accumulate in the middle, enter the human body through the food chain, and pose a threat to human health. International regulations on organotin residue limits in poultry meat, such as the positive list limits in the European Union and Japan...

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Problems solved by technology

[0004] There are no reports on the analysis methods of thes

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