A kind of β-tcp/pgs composite scaffold and its preparation method and application

A composite scaffold and porous scaffold technology, applied in medical science, prosthesis, etc., can solve the problems of poor toughness, β-TCP biological activity, limited improvement of β-TCP toughness and strength, etc., to achieve rapid repair and promote bone repair. , the effect of promoting vascularization

Inactive Publication Date: 2017-05-10
EAST CHINA UNIV OF SCI & TECH
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because of the inherently poor toughness of these polymers, there is limited improvement in toughness and strength for β-TCP
At the same time, the content of too high polymer material will affect the biological activity of β-TCP

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of β-tcp/pgs composite scaffold and its preparation method and application
  • A kind of β-tcp/pgs composite scaffold and its preparation method and application
  • A kind of β-tcp/pgs composite scaffold and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Synthesis and Purification of PGS Prepolymer

[0105] PGS prepolymers are synthesized by a two-step process.

[0106] Step 1: Under an argon atmosphere, react sebacic acid and glycerin at a molar ratio of 1:1 at 130°C for 24 hours;

[0107]The second step: the product of the first step was continued to react for 24 hours at 130° C. under vacuum conditions to obtain a PGS prepolymer.

[0108] Purification is then carried out. Firstly, the PGS prepolymer is dissolved in ethanol, the obtained emulsion is allowed to stand for a period of time, and the supernatant liquid is poured off. The obtained PGS prepolymer was placed in a dialysis bag and dialyzed to obtain a purified PGS prepolymer with a molecular weight of 12000 Da and a dispersion coefficient of 1.19.

[0109] Purification of PGS prepolymer 1 H-NMR such as figure 1 Shown in A. The proton peaks at 1.31, 1.62 and 2.35ppm mainly indicate the presence of sebacic acid units in the synthesized polymer chains, and t...

Embodiment 2

[0113] Synthesis of β-TCP Nanoparticles and Preparation of β-TCP Porous Scaffold

[0114] 0.4mol Ca(NO 3 ) 2 4H 2 O and 0.4mol(NH 4 ) 2 HPO 3 Dissolve in 200ml ultrapure water respectively, and stir rapidly for 30min to obtain Ca salt and P salt solutions. Add the Ca solution to the P solution drop by drop (1-2 drops / second), while maintaining constant stirring and adding the prepared ammonia water (2M) to adjust the pH value of the mixed solution to be stable at 7.0-7.5. After the dropwise addition was completed, the stirring was stopped, and the solution was allowed to stand for 2 hours to allow the solution to precipitate naturally, and the supernatant liquid was poured off. Then put the obtained precipitate into a centrifuge bottle, carry out centrifugation (6000r / min, 10min), wash with ultrapure water in the next step, ultrasonic (10min), continue to centrifuge, then repeat the water wash 2 times, and then wash it again with absolute ethanol After centrifugation, t...

Embodiment 3

[0121] Preparation of β-TCP / 10P-Unc Porous Scaffold

[0122] The prepared PGS prepolymer (PGS-1:1, prepared in Example 1) was formulated into a solution with a concentration of 10 mg / ml using absolute ethanol as a solvent. Place the β-TCP porous scaffold (0.2 g in weight) in a 24-well plate, add 2 ml of PGS solution to completely submerge the scaffold, and then place the porous plate in a fume hood for 3 to 5 hours to remove ethanol. Then the scaffold was freeze-dried at -50° C. under vacuum conditions for 24 hours to obtain the product β-TCP / 10P-Unc porous scaffold. The size of the pores is between 300-500 microns. The porosity is 90±2%. The maximum compressive strength is 0.02±0.01MPa, and the fracture deformation is 120±32%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
compressive strengthaaaaaaaaaa
particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses a Beta-TCP / PGS composite scaffold and a preparation method and application thereof. The Beta-TCP / PGS composite scaffold comprises PGS (poly glycerol sebacate) and a Beta-TCP (Tertiary Calcium Phosphate) porous scaffold. Particularly, the Beta-TCP / PGS composite scaffold is prepared by impregnating the Beta-TCP porous scaffold with a PGS prepolymer solution and then preforming thermal-crosslinking and drying. The invention further discloses a medicine composition and a tissue-engineered bone repair material, which comprise the composite bracket. Compared with a pure Beta-TCP porous scaffold, the Beta-TCP / PGS composite scaffold prepared by the invention shows excellent tenacity and compression strength, meanwhile has favorable biocompatibility and can be used as a bone repair material and a medicine carrier material.

Description

technical field [0001] The invention relates to a β-tricalcium phosphate / polymer material composite scaffold system for bone tissue repair and its preparation method and application, in particular to a β-tricalcium phosphate / polyglyceryl sebacate composite scaffold system and its preparation method and apply. Background technique [0002] β-tricalcium phosphate (β-TCP) is similar to the main inorganic components in human natural bone, has good biocompatibility and biodegradability and excellent osseointegration ability, and has high hardness and rigidity, which is convenient for acceleration Therefore, it has been widely used in bone tissue repair engineering, and it is also one of the most widely used bone repair materials so far. Among them, the β-TCP porous scaffold material, which is convenient for cells and ingrowth, has attracted extensive attention from researchers at home and abroad. However, the single β-tricalcium phosphate porous scaffold has problems such as hi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/12A61L27/38A61L27/54A61L27/56A61K47/34
Inventor 刘昌胜杨凯张婧袁媛李玉林马一帆
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap