A kind of strong loquat dew fingerprint quality control method
A powerful loquat extract and fingerprint technology, applied in the field of fingerprint analysis, can solve problems such as backwardness, and achieve the effect of scientific detection and stable quality
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Embodiment 1
[0031] (1) Accurately measure 25ml of the content of Qiangli loquat dew; place it in a separatory funnel, extract 4 times with water-saturated n-butanol, 25ml each time, combine the n-butanol solution, evaporate to dryness in a water bath, dissolve the residue with 50% methanol and Set the volume in a 5ml measuring bottle, shake well, filter with a 0.45 μm microporous membrane, and take the filtrate to obtain the product solution to be tested; (2) Repeat 6 times to obtain the fingerprint of the above solution to obtain 6 fingerprints , the acquisition method of fingerprint spectrum is: with 5g / L ammonium acetate aqueous solution A (adjust pH value to 7.5 with ammonia test solution) and acetonitrile B according to the mixed solution that certain volume ratio forms as mobile phase, chromatographic condition is: adopt Diamonsil C 18 Chromatographic column (150mm×4.6mm, 5μm); linear gradient elution program: within 0 to 30 minutes, the volume fraction of B in the mobile phase is 2%...
Embodiment 2
[0039] (1) Precisely measure 150ml of the content of the same batch of strong loquat dew, divide it into 6 parts, 25ml each; place them in a separatory funnel, extract 4 times with saturated n-butanol, 25ml each time, and combine n-butanol solution, evaporated to dryness in a water bath, the residue was dissolved with 50% methanol and fixed volume in a 5ml measuring bottle, shaken up, filtered with a 0.45 μm microporous membrane, and the filtrate was taken to obtain 6 parts of the product solution to be tested;
[0040] (2) Obtain the fingerprints of the above-mentioned 6 parts of product solutions to be tested, and obtain 6 fingerprints. The acquisition method is: with 5g / L ammonium acetate aqueous solution A (adjust the pH value to 7.5 with ammonia test solution) and acetonitrile B according to a certain The mixed solution of volume ratio composition is mobile phase, and chromatographic condition is: adopt Diamonsil C 18 Chromatographic column (150mm×4.6mm, 5μm); linear grad...
Embodiment 3
[0048] (1) Accurately measure 25ml of the content of Qiangli loquat dew; place it in a separatory funnel, extract 4 times with water-saturated n-butanol, 25ml each time, combine the n-butanol solution, evaporate to dryness in a water bath, dissolve the residue with 50% methanol and Set the volume in a 5ml measuring bottle, shake well, filter with a 0.45 μm microporous membrane, and take the subsequent filtrate to obtain the product solution to be tested;
[0049] (2) Place the solution of the product to be tested at room temperature for 0h, 3h, 6h, 9h, 12h, 18h, and 24h, then inject and analyze, and obtain 7 fingerprints. The results are shown in image 3 , see Table 3-1 and 3-2 for related data. Results The RSD of the retention time of each peak was less than 0.14%, and the peak area RSD of the peak with a single peak area greater than 0.5% of the total peak area was less than 1.72%. Taking the fingerprint spectrum analyzed at 0 time as a contrast, the chromatograms analyzed...
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