Method for storing biological samples and kit

A technology of biological samples and complexing agents, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of FTA card direct amplification technology limitations, unfavorable sample collection and transfer, pH value change of filter paper cards, etc. , to achieve fast, safe and effective storage, high sample transfer efficiency, and easy operation

Active Publication Date: 2015-05-27
SUZHOU NUHIGH BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. Low sample transfer efficiency: the design of the sample collection rod of the indicating FTA card is not conducive to sample collection and transfer, and the sample transfer efficiency is low during actual operation
[0009] 2. Limited direct amplification technology: Since the original intention of the FTA card design is based on the premise of nucleic acid separation and extraction, and with the development of nucleic acid amplification technology, direct amplification technology has begun to be widely used in forensic DNA analysis, making the FTA card There are certain limitations in direct amplification techniques
However, there is still a problem in this method, that is, during the storage of the filter paper card in the air, with the prolongation of the storage time and the storage conditions, ambient temperature, ambient humidity, and CO in the air, 2 The influence of content, which leads to the change of the pH value of the filter paper card, the pink sample storage card is very prone to fading
[0011] 4. High cost of use: FTA card is a patented product of Whatman, which is expensive and costly to use

Method used

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  • Method for storing biological samples and kit
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  • Method for storing biological samples and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1: The method for preserving biological samples according to the present invention preserves nucleic acids at room temperature

[0072] Disposable sampling tool: Medical cotton swab (Robust Medical)

[0073] Sample source: 9947 standard DNA solution (30ng / μL)

[0074] Functional solution: Tris at a concentration of 480mM pH 8.0-9.5, 30mM EDTA, 90mg / mL SDS, 60mM Na 2 SO 3 and 0.02% thymol blue

[0075] Filter paper card: Wohua filter paper (34mm×35mm)

[0076] Sample collection: put the disposable sampling tool in the EP tube containing 100 μL 9947 standard DNA solution to make the solution completely adsorbed; then put the disposable sampling tool adsorbed with the DNA solution in the EP tube containing 100 μL functional solution The solution is completely absorbed; remove the disposable sampling kit and transfer to a filter paper card. The filter paper cards were placed at room temperature for 0 days, 4 days, 8 days, 12 days, 16 days, 20 days, 24 days, ...

Embodiment 2

[0095] Embodiment 2: The method for preserving biological samples according to the present invention preserves nucleic acids at room temperature

[0096] Disposable sampling tool: Medical cotton swab (Robust Medical)

[0097] Sample source: 9947 standard DNA solution (30ng / μL)

[0098] Functional solution: Tris at a concentration of 240mM pH 8.0-9.5, 15mM EDTA, 45mg / mL SDS, 30mM Na 2 SO 3 and 0.02% thymol blue

[0099] Filter paper card: Wohua filter paper (34mm×35mm)

[0100] Sample collection: put the disposable sampling tool in the EP tube containing 100 μL 9947 standard DNA solution to make the solution completely adsorbed; then put the disposable sampling tool adsorbed with the DNA solution in the EP tube containing 100 μL functional solution The solution is completely absorbed; remove the disposable sampling kit and transfer to a filter paper card. The filter paper cards were left at room temperature for 0 days, 4 days, 8 days, 12 days, 16 days, 20 days, 24 days, an...

Embodiment 3

[0103] Embodiment 3: Antibacterial detection of the method for preserving biological samples according to the present invention

[0104] Disposable sampling tool: Medical cotton swab (Robust Medical)

[0105] Source of bacteria: XL10-gold Escherichia coli (engineering bacteria),

[0106] Functional solution composition:

[0107] Solution 1: Tris at a concentration of 240mM pH 8.0-9.5, 15mM EDTA, 45mg / mL SDS, 30mM Na 2 SO 3 and 0.02% thymol blue.

[0108] Solution 2: Tris at a concentration of 480mM pH 8.0-9.5, 30mM EDTA, 90mg / mL SDS, 60mM Na 2 SO 3 and 0.02% thymol blue.

[0109] Filter paper card: Wohua filter paper (34mm×35mm)

[0110] Sample collection: Put the disposable sampling tool in the EP tube containing 100 μL of bacterial suspension to make the solution completely adsorbed; then place the disposable sampling tool with adsorbed bacterial suspension in the EP tube containing 100 μL of different functional solutions, Allow the solution to absorb completely; re...

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PUM

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Abstract

The invention belongs to the technical field of biology and in particular discloses a method for storing biological samples and a kit. The method for storing the biological samples comprises the following steps: fully mixing a collection tool with adsorption of the biological samples with a functional solution, and transferring the mixed biological samples to a filter paper card for storage, wherein the functional solution comprises a buffering solution, a complexing agent, an antibacterial agent, a color indicator and an antioxidant. The method for storing the biological samples is simple and convenient to operate and high in sample transferring efficiency, and is capable of better killing bacteria and viruses. The collected biological samples are further mixed and subsequently transferred to the ordinary filter paper card; the purpose of quickly, safely and effectively storing the genetic materials in the biological samples can be achieved; meanwhile, the phenomenon of fading in long-term storage can be avoided.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method and kit for preserving biological samples, which are widely used in the diagnosis and monitoring of genetic diseases in biological, chemical and medical detection reactions, the establishment of biological banks, molecular diagnosis, PCR amplification , forensic, genetic identification, etc. Background technique [0002] Biological genetic material (GM), including human or animal physiological / pathological fluids (secretions, exudates, etc.); human or animal cell suspensions (blood, lymph, synovial fluid, semen, saliva, etc.); Physiological / pathological body fluids or cell suspensions of plants; extracts or suspensions of bacteria, fungi, plasmids, viruses, parasites, etc.; human or animal tissue (liver, kidney) extracts or tissue homogenates; DNA or RNA synthesis Process intermediates; chemically or biochemically synthesized DNA / RNA mixtures; anything else that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/68
Inventor 毕万里吴宁马静
Owner SUZHOU NUHIGH BIOTECH
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