Preparation method of phospholipid extract of chicken skin

The technology of chicken skin phospholipid and extract is applied in the field of preparation of chicken skin phospholipid extract, which can solve the problem of insufficient utilization of waste, and achieve the effects of improving brain function, promoting metabolism and simple operation.

Inactive Publication Date: 2015-06-03
NANJING UNIV OF FINANCE & ECONOMICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, my country's chicken product processing industry has developed rapidly, but the waste in chicken production has not been fully utilized

Method used

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  • Preparation method of phospholipid extract of chicken skin
  • Preparation method of phospholipid extract of chicken skin
  • Preparation method of phospholipid extract of chicken skin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation: Precisely weigh 50g of Nanjing native chicken breast body chicken skin, and cut it into pieces with scissors to about 4mm;

[0029] (2) Solvent preparation: the extract is chloroform:methanol prepared in a volume ratio of 2:1;

[0030] (3) Extraction of total lipids: add 300mL chloroform:methanol (2:1v:v) and extract in a constant temperature oscillator for 1h, let stand to collect the supernatant, add 200mL chloroform:methanol (2:1v:v) to extract the residue 1h, repeated 2 times, the temperature was controlled at about 35°C, the 3 supernatants were filtered and combined, layered with chloroform:methanol:distilled water (8:4:3v:v:v), and the lipid layer was collected;

[0031] (4) Drying: Concentrate the separated lipid layer with a rotary evaporator, and dry it in vacuum at 45°C to a constant weight to obtain the total lipid;

[0032] (5) Preparation of silica gel column: Weigh 10 g of silica gel in a beaker, activate at 120°C for 2 hours, add 40 ml o...

Embodiment 2

[0040] (1) Material preparation: Precisely weigh 10g of Xinjiang Baicheng oil chicken breast body chicken skin, and cut it into pieces with scissors to about 4mm;

[0041] (2) Solvent preparation: the extract is chloroform: methanol is prepared in a volume ratio of 2:1;

[0042] (3) Extraction of total lipids: Add 60 mL of chloroform:methanol (2:1v:v) and extract on a constant temperature oscillator for 1 hour, let stand to collect the supernatant, add 40mL of chloroform:methanol (2:1v:v) to the residue for extraction 1h, repeated 2 times, the temperature was controlled at about 35°C, the 3 supernatants were filtered and combined, layered with chloroform:methanol:distilled water (8:4:3v:v:v), and the lipid layer was collected;

[0043] (4) Drying: Concentrate the separated lipid layer with a rotary evaporator, and dry it in vacuum at 45°C to a constant weight to obtain the total lipid;

[0044] (5) Preparation of silica gel column: Weigh 10 g of silica gel in a beaker, activa...

Embodiment 3

[0052] (1) Preparation: Precisely weigh 25g of Nanjing native chicken breast body chicken skin, and cut it into pieces with scissors to about 4mm;

[0053] (2) Solvent preparation: the extract is chloroform: methanol is prepared in a volume ratio of 2:1;

[0054] (3) Extraction of total lipids: add 150mL chloroform:methanol (2:1v:v) and extract in a constant temperature oscillator for 1h, let stand to collect the supernatant, add 100mL chloroform:methanol (2:1v:v) to the residue for extraction 1h, repeated 2 times, the temperature was controlled at about 35°C, the 3 supernatants were filtered and combined, layered with chloroform:methanol:distilled water (8:4:3v:v:v), and the lipid layer was collected;

[0055] (4) Drying: Concentrate the separated lipid layer with a rotary evaporator, and dry it in vacuum at 45°C to a constant weight to obtain the total lipid;

[0056] (5) Preparation of silica gel column: Weigh 10 g of silica gel in a beaker, activate at 120°C for 2 hours, ...

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Abstract

The invention discloses a preparation method of a phospholipid extract of chicken skin and belongs to the technical field of food biology. An extraction method of functional phospholipids, provided by the invention, comprises the steps of preparation of the chicken skin, solvent extraction of total lipid, drying, silica gel column separation, concentration, phospholipid detection and the like. The extract obtained by using the method is rich in multiple functional phospholipids such as cephalin, lecithin and sphingomyelin, a phospholipid side chain contains unsaturated fatty acid, and the human health is facilitated. The method is simple and convenient to operate, low in cost and higher in extraction rate, and can be widely applied to preparation of the functional phospholipids in the chicken skin. The extract can be widely applied as a raw material of healthcare food.

Description

1. Technical field [0001] The invention relates to a preparation method of chicken skin phospholipid extract, which belongs to the field of food biotechnology. 2. Background technology [0002] Functional lipids include phospholipids, glycolipids and other derivatives, which are important components of human biomembranes. Most of these functional lipid components are polar lipids. The intake of phospholipids can inhibit thrombus, activate defense function, promote phagocytosis, participate in relevant metabolism in the body, produce a series of physiologically active substances, and regulate physiological functions. Representative phospholipids include cephalin, lecithin, sphingomyelin, phosphatidylserine, plasmalogen, etc. These phospholipids play an important role in human life activities, and regular use can promote fat metabolism and improve brain function. Diseases such as cerebrovascular disease, neurological disease, diabetes, Alzheimer's disease (senile dementia) a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J7/00A23L1/29A23L1/312A23L13/20A23L33/00
Inventor 方勇潘雨时热娜古丽-木沙陈悦夏季王红盼胡秋辉马宁杨文建裴斐
Owner NANJING UNIV OF FINANCE & ECONOMICS
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