A kind of ruminant feed biological enhancer and preparation method thereof

A biological enhancement, ruminant technology, applied in the direction of biochemical equipment and methods, animal feed, microorganism-based methods, etc., can solve the problems of unstable live bacteria content, different fermentation conditions, long drying time, etc., to improve physiological functions. , does not pollute the environment, enhances the effect of absorption and utilization

Active Publication Date: 2017-07-07
内蒙古柯宏生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1) At present, similar products are all dried. If the drying temperature is too high, it will easily lead to the inactivation of the strain. However, if the drying temperature is low, the drying time will be long and consume energy.
[0007] 2) Although most of the similar products are currently fermented by the composite bacteria of Bacillus, lactic acid bacteria and yeast, due to the different fermentation conditions, simultaneous fermentation will have mutual influence
[0009] 4) The content of live bacteria in the product is unstable and the price is high and it is difficult to promote

Method used

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  • A kind of ruminant feed biological enhancer and preparation method thereof
  • A kind of ruminant feed biological enhancer and preparation method thereof
  • A kind of ruminant feed biological enhancer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Preparation method of ruminant feed bioenhancer of the present invention

[0031] Experimental group 1 press Prepare ruminant feed bioenhancer of the present invention according to the following method:

[0032] a) Prepare a fermentation medium, by weight: 85%-95% of carbon source, 1%-2% of nitrogen source, 1%-3% of growth promoter, and water as the balance, pulverize and mix the above ingredients ,spare;

[0033] b) Inoculate Candida utilis in the fermentation medium obtained in step a), perform solid fermentation culture, the inoculum size is 10%, and ferment and cultivate at 32° C. for 24 hours;

[0034] c) Inoculate Lactobacillus plantarum in the fermentation medium obtained in step b), perform anaerobic solid fermentation culture, and take samples to detect OD every 4h 600 , inoculum size 6%, anaerobic fermentation culture at 30°C for 20-24h;

[0035] d) to be OD 600 Enter the logarithmic growth phase, inoculate Bacillus subtilis in the fermentat...

Embodiment 2

[0044] Example 2 The effect of the preparation sequence of the ruminant feed bioenhancer of the present invention on the bacteria content and vitamin content.

[0045] The bacteria content and vitamin B group content of each group of ruminant feed bioenhancers prepared in Example 1 were tested in parallel 5 times for each group, and the average value was taken. The results are shown in Table 1 below and were statistically analyzed.

[0046] Table 1 The number of viable bacteria and the content of vitamin B family in the bioenhancers of ruminant feed in each group

[0047]

[0048] From the results of this experiment, it can be seen that there are significant differences in the number of viable bacteria and the content of vitamin B family between the experimental group and the control group, and the different inoculation sequences of the strains will have a huge impact on the yield of the strains and the yield of vitamins, especially vitamin B, It is a key factor for ruminants...

Embodiment 3

[0049] The influence of embodiment 3 different inoculum sizes on the quality of feed bioenhancer

[0050] Experimental group 2-4 was prepared according to the method of Experimental Example 1, and the inoculation amount was shown in Table 2 below:

[0051] Table 2 Inoculation volumes used in experimental groups 1-4

[0052]

[0053]

[0054] The bacteria content and vitamin B group content of each group of ruminant feed bioenhancers prepared in Example 3 were tested in parallel 5 times for each group, and the average value was obtained. The results are shown in Table 3 below and statistically performed.

[0055] Table 3 Experimental results of experimental groups 1-4

[0056]

[0057] From the experimental results, it can be seen that there are significant differences between the experimental group 1 and the experimental groups 2, 3 and 4 in the number of viable bacteria and the content of vitamin B family. It can be seen that different inoculum volumes will also af...

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Abstract

The invention relates to a ruminant feed bioenhancer and a preparation method thereof, which comprises the following steps: a) preparing a fermentation medium, by weight: a carbon source of 85%-95%, a nitrogen source of 1%-2%, The growth promoter is 1%-3%, and the balance is water. The above-mentioned ingredients are crushed, mixed, and set aside; b) inoculating Candida utilis in the fermentation medium obtained in step a), and solid fermentation culture; c) in step a) b) Lactobacillus plantarum is inoculated in the obtained fermentation medium, and anaerobic solid fermentation is cultivated, and OD600 is detected by sampling every 4 hours; d) when OD600 enters the logarithmic growth phase, Bacillus subtilis is inoculated in the fermentation medium obtained in step c), and solid fermentation is carried out. nourish. The biological feed prepared by the method can improve the rumen micro-ecological balance of ruminants, improve feed conversion rate and body immunity, and the product has a special sour-sweet smell, good palatability, and easy for animals to eat.

Description

technical field [0001] The invention belongs to the technical field of application of rumen microorganisms in ruminants, and in particular relates to a feed bioenhancer for ruminants and a preparation method thereof. Background technique [0002] With the rapid development of dairy farming and mutton sheep fattening industry, the production performance of animals continues to increase, and the widespread use of antibiotics leads to endogenous infections and drug resistance in animals; Excreted from the body, the micro-ecological balance of the normal flora in the intestinal tract of animals is destroyed, and the production performance of animals is reduced, thereby affecting the economic and social benefits of the breeding industry. [0003] Using the theory and technology of rumen micro-ecological balance in ruminants, a variety of beneficial microorganisms are cultivated to prepare compound microbial agents, which can be used in ruminant nutrition to supplement the number ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/10A23K20/00C12N1/20C12N1/16C12R1/125C12R1/72
Inventor 宁瑛
Owner 内蒙古柯宏生物科技有限公司
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