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A kind of gold nanoparticle complex with the effect of inhibiting nerve cell apoptosis and its application

A nerve cell apoptosis and gold nanoparticle technology, applied in gold nanoparticle complexes and their application fields, can solve the problems of no discovery, and achieve the effects of small size, easy degradation and low toxicity

Active Publication Date: 2017-12-29
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the research on the delivery and transfection of nucleic acids by gold nanoparticles, functionalized gold nanoparticles are currently used to absorb nucleic acids to transfect African green monkey kidney cells, HeLa cells, breast cancer cells and melanoma cells, to inhibit virus infection, Dermatological treatment, etc., but there is no research on the use of functionalized gold nanoparticles to adsorb plasmid DNA for inhibiting nerve cell apoptosis or inhibiting apoptosis in Parkinson's disease cell models

Method used

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  • A kind of gold nanoparticle complex with the effect of inhibiting nerve cell apoptosis and its application
  • A kind of gold nanoparticle complex with the effect of inhibiting nerve cell apoptosis and its application
  • A kind of gold nanoparticle complex with the effect of inhibiting nerve cell apoptosis and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Preparation of a gold nanoparticle complex capable of inhibiting nerve cell apoptosis

[0057] 1. Preparation of dithiodisuccinimidyl propionate (DTSP):

[0058] Dissolve 4.2504 g (0.02 mol) of 3,3'-dithiodipropionic acid (DTPA) and 4.6036 (0.04 mol) of N-hydroxysuccinimide (HOSu) in 20 mL of dimethyl Formamide; Dissolve 4.1266 g (0.02 mmol) of dicyclohexylcarbodiimide in 20 mL of dimethylformamide; add it dropwise to the reaction mixture under stirring, react in an ice bath for 24 hours, then filter; then Dilute with ethyl acetate, add dilute hydrochloric acid to remove the remaining dicyclic urea. Then the solvent was removed by evaporation to obtain a solid product, which was washed three to five times with dilute hydrochloric acid, then ethyl acetate was added to remove the aqueous hydrochloric acid solution, and the solvent was removed by rotary evaporation to obtain the product, namely DTSP.

[0059] 2. Preparation of thiolated chitosan (TCTS):

[006...

Embodiment 2

[0067] Example 2 Infrared Spectroscopy Detection to Characterize the Synthesized Gold Nanoparticle Composite

[0068] 1. Dry DTPA, HOSu, DTSP, CTS, TCTS, GNP, CTS@GNP and CTS@GNP-GNP, then put them into a mortar, add a certain amount of KBr, and grind the mixture evenly until the particle size is less than 2 μm , so as not to be affected by scattered light, then put it into a dryer for drying treatment, press the mixture into a transparent sheet with a pressure of about 10MPa on a hydraulic press, and measure it on the machine.

[0069] 2. Results

[0070] The test results are attached figure 1 shown. figure 1 It is the infrared spectrogram of the corresponding reactants and products characterized by Fourier transform infrared spectrometer. Spectrum a is at 1698cm -1 There is a strong absorption peak, which is the C=O stretching vibration on the carboxyl group of 3,3'-dithiodipropionic acid; Spectrum b is at 1651 cm -1 、1705 cm -1 、1780 cm -1 The absorption peak is the ...

Embodiment 3

[0071] Example 3 Characterization of the synthesized gold nanoparticle composite by Raman spectroscopy

[0072] 1. Dry DTPA, HOSu and DTSP, and then take appropriate amount of samples on glass slides for detection on the machine.

[0073] 2. Results

[0074] The test results are attached figure 2 shown. figure 2 It is a Raman spectrum characterized by a Raman spectrometer to the reactant 3,3'-dithiodipropionic acid, N-hydroxysuccinimide and the product DTSP. Spectrum A at 659 cm -1 Raman peak at and 500 cm -1 The Raman peaks at are caused by the stretching vibrations of the C-S chain group and the S-S chain group in 3,3'-dithiodipropionic acid respectively, and there is no such Raman peak in spectrum B; while spectrum C is in 665cm -1 and 507 cm -1 The Raman peak at is caused by the stretching vibration of the C-S chain group and the S-S chain group in DTSP, which can prove the role of 3,3'-dithiodipropionic acid and N-hydroxysuccinimide in the dehydrating agent DCC ...

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Abstract

The invention provides a gold nanoparticle compound capable of inhibiting nerve cell apoptosis and application thereof. The gold nanoparticle complex is modified with thiolated chitosan (TCTS) on the surface of gold nanoparticles (GNP), then electrostatically adsorbs the pDNA that interferes with the synthesis of α-synuclein, and finally adopts the method of photografting to graft nerve cells. growth factor (NGF). In the present invention, the successfully synthesized gold nanoparticle complex is applied to nerve cells, and it is found that the complex can significantly inhibit the apoptosis of the cells, and has an inhibitory effect on the apoptosis of Parkinson's disease in vitro cell models. Another method and new drug for the treatment of Parkinson's disease that emerged after treatment and chemotherapy have great guiding significance for the treatment and research of this disease.

Description

technical field [0001] The invention belongs to the field of gene therapy drugs. More specifically, it relates to a gold nanoparticle complex capable of inhibiting nerve cell apoptosis and its application. Background technique [0002] Parkinson's disease (PD) is a progressive neurological disorder characterized by degeneration of dopaminergic neurons in the nigrostriatum of the midbrain and a decrease in the neurotransmitter dopamine. When the synthesis of dopamine decreases, the function of inhibiting acetylcholine decreases, and the imbalance between the two results in "paralysis agitans". According to statistics, for PD, the incidence rate is lower for those under 45 years old, 3.1% for those aged 75-85, and 4.3% for those over 85 years old. Currently, proteins related to Parkinson's disease are involved in lipid-vesicle dynamics (α-synuclein, α-synuclein), ubiquitin-proteasome system (parkin and UCHL1), mitogen-activated protein kinase signaling pathway (LRRK2), oxid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K38/18A61K9/14A61K47/02A61K47/36A61P25/28A61P25/16
Inventor 关燕清陈晓辉
Owner SOUTH CHINA NORMAL UNIVERSITY
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