Microbial water purifying agent for sea cucumber cultivation water bodies

A technology for aquaculture water and water purifying agent, applied in biological water/sewage treatment, water/sludge/sewage treatment, chemical instruments and methods, etc. disease capacity, reduce pathogens, and improve the effect of degradation

Inactive Publication Date: 2015-06-03
DALIAN SANKE BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many kinds of microorganisms have been applied to the purification of aquaculture water, and good results have been obtained. However, in order to obtain better precipitation effects, the aquaculture water purifier based on the characteristics of the aquaculture water itself needs further research and development.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A microbial water purifier for sea cucumber cultivation water, comprising a compound microbial liquid and auxiliary materials, wherein the compound microbial liquid is a mixture of Bacillus subtilis liquid, Bacillus lateral sporosum liquid, Lactobacillus acidophilus liquid, and yeast liquid; The auxiliary material is a mixture of bentonite and soybean meal.

[0015] Described water purifying agent is prepared as follows:

[0016] (1) Inoculate Bacillus subtilis in 100ml liquid LB medium, culture at 30°C for 16 hours, and the effective number of viable bacteria is 2×10 7 Individual / ml of Bacillus subtilis liquid;

[0017] (2) Inoculate Bacillus lateralis in 100ml of liquid LB medium, culture at 28°C for 24 hours, and the effective number of viable bacteria is 3×10 7 Individual / ml of Bacillus lateralis solution;

[0018] (3) Inoculate Lactobacillus acidophilus in 100ml liquid LB medium, culture at 28°C for 32 hours, and the effective number of viable bacteria is 1.7×10...

Embodiment 2

[0023] A microbial water purifier for sea cucumber cultivation water, a microbial water purification agent for sea cucumber cultivation water, comprising a compound microbial liquid and auxiliary materials, wherein the compound microbial liquid is Bacillus subtilis liquid, Bacillus lateralis liquid, yeast The mixture of bacteria liquid; the auxiliary material is bentonite.

[0024] Described water purifying agent is prepared as follows:

[0025] (1) Inoculate Bacillus subtilis in 100ml liquid LB medium, culture at 30°C for 16 hours, and the effective number of viable bacteria is 2×10 7 Individual / ml of Bacillus subtilis liquid;

[0026] (2) Inoculate Bacillus lateralis in 100ml of liquid LB medium, culture at 28°C for 24 hours, and the effective number of viable bacteria is 3×10 7 Individual / ml of Bacillus lateralis solution;

[0027] (3) Inoculate the yeast into 100ml liquid PDA liquid medium, culture at 32°C for 30 hours, and the effective number of viable bacteria is 4×1...

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PUM

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Abstract

The invention discloses a microbial water purifying agent for sea cucumber cultivation water bodies, which comprises the following components in parts by mass: 30-40 parts of composite microbial bacterium solution and 30-80 parts of auxiliary material. The composite microbial bacterium solution is one or mixture of more of Bacillus subtilis solution, Bacillus lateraporus solution, Lactobacillus acidophilus solution and microzyme solution, and the effective viable count of each bacterium solution is 10<6>10<8> / ml; and the auxiliary material is one or mixture of more of bentonite, bean pulp and corn flour. The water purifying agent has favorable degradation actions on ammonia nitrogen, nitrites and other pollutants in the sea cucumber cultivation water body, and is capable of effectively inhibiting the pathogenic microorganism from growth, reducing the pathogens and enhancing the disease resistance of the sea cucumbers. The strains with high water purification efficiency and stable performance are reasonably compounded to maximally display the functional complementary actions of the strains; and the microbial water purifying agent has the advantages of reasonable formula and low price, and is suitable for popularization.

Description

technical field [0001] The invention relates to a water purifier for aquaculture water, in particular to a microbial water purifier for sea cucumber aquaculture water. Background technique [0002] In recent years, the nutritional value of sea cucumbers and the large demand of the market have led to the rapid development of sea cucumber artificial breeding. At present, in the traditional feeding process of sea cucumbers, due to the low bait utilization rate, residual bait, and defecation of farmed animals, the seawater will be turbid and eutrophic, which will lead to a large number of plankton in the water and destroy the growth environment of sea cucumbers. Sea cucumbers grow slowly and are susceptible to disease. In addition, the increase in the degree of eutrophication in the water body will lead to excessive ammonia nitrogen and nitrite, which will have a toxic effect on aquatic animals. Therefore, solving the problems of eutrophication and ammonia nitrogen pollution i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F3/34C02F3/347
Inventor 崔国臣顾艳丽崔昌娜周悦
Owner DALIAN SANKE BIO ENG
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