Broth for preventing and treating ralstonia solanacearum and preparation method thereof

A technology of tobacco bacterial wilt and fermentation liquid, which is applied in the field of agricultural microorganism application, can solve problems such as weak research on applied technology, failure to achieve control effects, environmental pollution, etc., achieve strong pertinence, control tobacco bacterial wilt, and enhance prevention and control effect of disease

Inactive Publication Date: 2017-03-01
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, the development of biological control agents for bacterial wilt is relatively lagging behind, and there are few effective agents. In addition, the large-scale use of chemical pesticides for prevention and control will cause pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides has caused pathogenic bacteria to produce drug resistance
On the other hand, the research on the application technology of the agents used to control this bacterial wilt is also very weak
Due to the diversity and synchronous evolution of pathogenic bacteria, a single antagonistic bacterial strain cannot achieve an ideal control effect. Therefore, many biological functions of biocontrol bacteria need to rely on the synergy between two or more strains of bacteria to exert a better effect. good effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]A fermented liquid for preventing and treating bacterial wilt of tobacco is characterized in that the raw materials for preparing the fermented liquid are in parts by weight: 30 parts of Streptomyces pleochroicus seed liquid, 30 parts of Streptomyces recififera seed liquid, fermented by Bacillus suffolus 30 parts of Brevibacillus mesosporus fermentation broth, 40 parts of Trichoderma maroon seed liquid, 30 parts of sugarcane molasses, 50 parts of soybean molasses, 50 parts of cotton meal powder, 50 parts of compound amino acid raw powder, 50 parts of potassium fulvic acid Parts, 6 parts of calcium carbonate, 4 parts of magnesium sulfate, 800 parts of water.

[0034] The preparation method of the fermented liquid for preventing and treating tobacco bacterial wilt comprises the following steps:

[0035] (1) Add in turn to the fermentation tank: 30 parts of sugarcane molasses, 50 parts of soybean molasses, 50 parts of cotton meal powder, 6 parts of calcium carbonate, 4 part...

Embodiment 2

[0056] Anti-efficacy test of fermented liquid of the present invention in greenhouse potted plants

[0057] The tobacco variety is China Tobacco 98, sow the germinated seeds to the seedling trays, and transplant them into pots after about 12 days.

[0058] One plant per pot was placed in a greenhouse at 26°C for cultivation. After transplanting 4 days, every plant seedling waters the 100 times dilution of the fermented liquid that 50ml embodiment 1 prepares, irrigates root and inoculates tobacco solanacearum after 24h, inoculation concentration 10 7 cfu / mL, 20ml per plant. Plants treated with 72% agricultural streptomycin were used as pesticide control, and plants treated with fermentation medium and water were used as blank control. There were 20 seedlings per treatment, and each treatment was repeated 3 times. After the onset of the disease, the incidence was investigated every day, and the incidence and disease index were counted, until the incidence of the clear water c...

Embodiment 3

[0071] Embodiment 3, fermented liquid of the present invention is to tobacco bacterial wilt treatment effect in field

[0072] Location: A grower in Tantou Town, Longhui County, Hunan Province

[0073] Pathogenic tobacco bacterial agent spraying treatment: to the diseased strain that occurs, every strain of diseased strain pours 100 times of dilutions of the fermented liquid that 50ml embodiment 1 prepares; Pesticide streptomycin group: handle with 72% agricultural streptomycin root irrigation plants; the blank control only sprayed water on the pathogenic strains.

[0074] Results: For the tobacco leaves sprayed with water, the withering rate reached 55% after 3 days, and 96% withered after 6 days. For applying the pesticide streptomycin group, after 3 days, the withering rate reached 23%, and after 6 days, 35% withered, and about 40% of the diseased plants were controlled; Days later, the disease state of all diseased plants was initially controlled, and 95% of the diseased...

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PUM

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Abstract

The invention discloses a broth for preventing and treating ralstonia solanacearum and a preparation method thereof. According to the invention, different biocontrol fungus are combined, then mixed according to different proportions and fermented, and a stable and effective combination is screened, the screened combination can effectively control the ralstonia solanacearum through a root watering application method; the broth for preventing and treating ralstonia solanacearum is prepared by fermenting streptomyces pluricolorescens seed liquid, streptomyces recifensis seed liquid, a bacillus safensis broth, a brevibacilluscentrosporus broth, trichoderma rufobrunneum seed liquid, cane molasses, soybean molasses, cottonseed meal powder, composite amino acid raw powder, potassium fulvic acid, calcium carbonate, magnesium sulfate, and 800 parts of water.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganism application, in particular to a fermented liquid for preventing and treating tobacco bacterial wilt and a preparation method thereof. Background technique [0002] Soil-borne diseases are devastating diseases that harm tobacco production, and tobacco bacterial wilt is the most serious in our country. Tobacco bacterial wilt (Tobacco Bacterial Wilt) is one of the devastating diseases on tobacco caused by Ralstonia solanacearum, which causes huge economic losses every year. For the prevention and treatment of this disease, there is no effective biological control agent and control agent technology at present. On the one hand, the development of biological control agents for bacterial wilt is relatively lagging behind, and there are few effective agents. In addition, the large-scale use of chemical pesticides for prevention and control will cause pesticide residues and environmenta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01N63/02A01P3/00
CPCA01N63/10A01N63/30A01N2300/00
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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