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Membrane-separation-type Culture Device, Membrane-separation-type Culture Kit, Stem Cell Separation Method Using Same, And Separation Membrane

A culture device and stem cell technology, applied in chemical instruments and methods, semi-permeable membrane separation, non-embryonic pluripotent stem cells, etc., can solve problems that have not yet been established

Inactive Publication Date: 2015-06-03
NAT CENT FOR GERIATRICS & GERONTOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is, a separation membrane module that satisfies blood compatibility with a high-performance separation membrane has not yet been established.

Method used

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  • Membrane-separation-type Culture Device, Membrane-separation-type Culture Kit, Stem Cell Separation Method Using Same, And Separation Membrane
  • Membrane-separation-type Culture Device, Membrane-separation-type Culture Kit, Stem Cell Separation Method Using Same, And Separation Membrane
  • Membrane-separation-type Culture Device, Membrane-separation-type Culture Kit, Stem Cell Separation Method Using Same, And Separation Membrane

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no. 1 Embodiment approach ）

[0096] The membrane sorting incubator 1 according to the first embodiment of the present invention is composed of an upper structure 10 and a lower structure 13 . The upper structure 10 is a structure for adding the medium 100 containing the sample cells 200 or the sample tissue and holding the sample cells 200 or the sample tissue on the separation membrane 12 . On the other hand, the lower structure 13 is a structure for adding a medium 300 containing a migration factor (not shown) and receiving migrated stem cells.

[0097] The upper structure 10 is a container composed of a side surface 11 and a circular bottom surface, and the bottom surface is formed of a separation membrane 12 having a plurality of holes 121 . The upper structure 10 only needs to be able to accommodate the culture medium 100 and the sample cells 200 or sample tissues inside. In the present specification, the sample cells refer to cells that have not been sorted from tissues in which adhesion between ce...

no. 3 approach

[0133] According to a third embodiment, the present invention is a membrane sorting culture device provided with a lid structure. image 3 It is a conceptual diagram showing the structure of the membrane sorting incubator 3 of this embodiment. The membrane sorting culture device 3 includes a cover structure 34 in addition to the upper structure 30 and the lower structure 33 .

[0134] In the present embodiment, the upper structure 30 is one container composed of a shaft-shaped portion having a circular bottom surface and a truncated conical portion having an opening. The upper structure body 30 is configured such that the diameter of the opening is larger than the diameter of the bottom surface. The opening of the truncated conical portion is provided with a holding mechanism 35 constituted by a flange protruding outward.

[0135] The lower structure 33 shown in the figure is a cylindrical member having a circular bottom surface and an opening with the same diameter. A groove...

no. 8 approach

[0167] According to an eighth embodiment, the present invention is a separation membrane.

[0168] The separation membrane of the present embodiment includes a base film and a functional layer, the base film is composed of a hydrophobic polymer, and the functional layer is selected from vinylpyrrolidone-based polymers, polyethylene glycol-based polymers, and vinyl alcohol-based polymers. A functional layer formed by covalently bonding one or more hydrophilic polymers in the polymer to the surface of the aforementioned substrate membrane, and the weight of the hydrophilic polymer constituting the aforementioned functional layer is equal to the total weight of the aforementioned separation membrane. 1.5% by weight to 35% by weight.

[0169] In the present invention, membrane surface modification by covalent bonding is preferred because pores of the membrane may be clogged or eluted in an aqueous culture solution, which may adversely affect cells due to residual organic solvents....

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Abstract

A membrane separation culture device 1, which comprises an upper structure 10 constituted with a vessel comprising a membrane 12 having, in at least a portion thereof, pores 121 that allow stem cells to permeate therethrough, and a lower structure 13 constituted with a vessel that retains a fluid in which the membrane of the upper structure is immersed; a membrane separation culture kit comprising the membrane separation culture device 1 and cell migration factor(s); a method for separating stem cells, which comprises a step of dispersing test cells or test tissues on the membrane 12 of the upper structure 10, a step of filling the lower structure 13 with a medium containing the cell migration factor(s), and a step of allowing the membrane 12 of the upper structure 10 to come into contact with the medium in the lower structure 13; and a separation membrane comprising a base material membrane consisting of a hydrophobic polymer and a functional layer formed by allowing one or more hydrophilic polymers selected from a vinyl pyrrolidone polymer, a polyethylene glycol polymer and a vinyl alcohol polymer to bind to the surface of the base material membrane via a covalent bond, wherein the weight percentage of the hydrophilic polymer(s) constituting the functional layer is 1.5% to 35% based on the total weight of the separation membrane.

Description

[0001] This application is the invention patent application No. PCT / JP2012 / 058637 filed on March 30, 2012 entitled "Membrane Sorting Culture Device, Membrane Sorting Culture Kit, Stem Cell Sorting Method Using It, and Separation Membrane" The divisional application of the original application entered the Chinese national phase and the national application number obtained was 201280016688.1. technical field [0002] The present invention relates to a membrane sorting culture device for sorting stem cells and dental pulp stem cells of any biological species, a membrane sorting culture kit, and a stem cell sorting method using the same. In particular, the present invention relates to a membrane sorting culture device for sorting dental pulp stem cells or mesenchymal stem cells filled in root canals for pulp regeneration, a membrane sorting culture kit, and a stem cell sorting method using the same. The present invention also relates to a separation membrane, a surface modificatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06C12N5/074C12N5/0775
CPCB01D63/087B01D67/0093B01D71/50C12M23/12C12M25/04C12N5/0664C12M41/12C12N5/0663C12N5/0667B01D2315/06C12M29/04C12M47/04C12M3/06C12N15/09B01D67/00933B05D1/18B05D3/068
Inventor 中岛美砂子庵原耕一郎山田和正岛垣昌明长部真博
Owner NAT CENT FOR GERIATRICS & GERONTOLOGY
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