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Multi-index component content measuring method for roots of common peonies and honeysuckles in Chinese herbal medicine compound preparation

A technology of compound preparation and determination method, which is applied in the field of medicine, can solve the problems that the determination of paeoniflorin content and caffeoylquinic acid content cannot be realized at the same time, and achieve the effects of shortening detection time, saving analysis time and accurate analysis data

Active Publication Date: 2015-06-24
SHANDONG INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

This document is measured at a wavelength of 327nm, but paeoniflorin has no absorption at around 327nm, and its content determination generally uses its maximum absorption wavelength of 230nm, so the content determination of paeoniflorin cannot be realized at the same time under the conditions of this document
At present, there is no method for simultaneous content determination of paeoniflorin and the above-mentioned 6 caffeoylquinic acids in the preparation in the prior art

Method used

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  • Multi-index component content measuring method for roots of common peonies and honeysuckles in Chinese herbal medicine compound preparation
  • Multi-index component content measuring method for roots of common peonies and honeysuckles in Chinese herbal medicine compound preparation
  • Multi-index component content measuring method for roots of common peonies and honeysuckles in Chinese herbal medicine compound preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0048] Experimental example 1 content determination experiment

[0049] 1. Instruments, reagents and test samples

[0050] Agilent 1200 HPLC (including G1322A degasser, G1311A quaternary pump, G1329A autosampler, G1316A column thermostat, G1314B VWD detector, ChemStation workstation); Sartorius CP225D electronic balance (Germany Sartorius Group) .

[0051] Reference substances chlorogenic acid (5-O-caffeoylquinic acid) and paeoniflorin, batch numbers 110753-201314, 110736-201337, contents 96.6%, 94.9%, were purchased from China National Institutes for Food and Drug Control; Reference substance 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 3, 4-O-dicaffeoylquinic acid, batch numbers are ps100603-07, ps100512-06, ps100623-01, ps100623-02, ps100623-03, all purchased from Chengdu Pusi Biotechnology Co., Ltd., purity ≥ 98%; Methanol and acetonitrile are chromatographically pure, and phosphoric acid is analytically pur...

Embodiment 1

[0118] Embodiment 1: A kind of raw material composition is honeysuckle 700g, Radix Paeoniae Rubra 700g, Mianma Guanzhong 233g, make the traditional Chinese medicine compound preparation Kanggan granule of total amount 1000g The determination method of multi-index component content of Radix Radix Radix Radixae Radix and Lonicerae Lonicerae, the steps are as follows:

[0119] Determination according to the high performance liquid chromatography of appendix VID of "Chinese Pharmacopoeia" 2010 edition;

[0120] Chromatographic conditions and system suitability test:

[0121] Octadecylsilane bonded silica gel is used as filler; acetonitrile is used as mobile phase A, phosphoric acid solution with a volume ratio of 0.4% is used as mobile phase B, and gradient elution method is adopted: 0min→14min→22min→23min→38min →39min; Acetonitrile: 8%→16%→16%→24%→24%→8%, phosphoric acid solution with a volume ratio of 0.4%: 92%→84%→84%→76%→76%→92% ; Detection by wavelength switching: 0~22min, d...

Embodiment 2

[0126] Embodiment 2: A kind of raw material medicine composition is honeysuckle 1500g, red peony root 1500g, Mianma Guanzhong 500g, make the traditional Chinese medicine compound preparation Kanggan capsule of total amount 450g The determination method of multi-index component content of red peony root and honeysuckle flower, the steps are as follows:

[0127] Determination according to the high performance liquid chromatography of appendix VID of "Chinese Pharmacopoeia" 2010 edition;

[0128] Chromatographic conditions and system suitability test:

[0129] Octadecylsilane bonded silica gel is used as filler; acetonitrile is used as mobile phase A, phosphoric acid solution with a volume ratio of 0.4% is used as mobile phase B, and gradient elution method is adopted: 0min→14min→22min→23min→38min →39min; Acetonitrile: 8%→16%→16%→24%→24%→8%, phosphoric acid solution with a volume ratio of 0.4%: 92%→84%→84%→76%→76%→92% ; Detection by wavelength switching: 0~22min, detect 3-O-caff...

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Abstract

The invention discloses a multi-index component content measuring method for the roots of common peonies and honeysuckles in a Chinese herbal medicine compound preparation. The multi-index component content measuring method has the advantages that a chromatographic condition is used for measuring content of paeoniflorin and six caffeoylquinic acids in the Chinese herbal medicine compound preparation comprising the roots of the common peonies and the honeysuckles, simplicity and convenience in operation are achieved, and analysis data are accurate; gradient elution conditions are reasonable, and when both chromatographic peak shapes and separation degrees of target components are considered, detection time is shortened greatly; analysis time of analysts is saved due to simultaneous detection of the seven components, and working efficiency is improved; the proportion of organic phases in mobile phases is low, testing cost is lowered, and environmental pollution is reduced.

Description

technical field [0001] The invention relates to a method for determining the content of a compound Chinese medicine preparation, in particular to a method for determining the content of Radix Paeoniae Rubra and Honeysuckle in a compound Chinese medicine preparation, and belongs to the technical field of medicine. Background technique [0002] Kanggan series preparations (Kanggan Granules and Kanggan Capsules) are composed of 3 herbs including honeysuckle, red peony root, and Mianmaguanzhong. Cold caused by exogenous wind-heat. Kanggan granule implements the standard of the first supplement of the 2010 edition of "Chinese Pharmacopoeia"; Kanggan capsule implements the National Drug Standard WS of the State Drug Administration 3 -225(X-215)-2002Z. Standard items include: TLC identification of chlorogenic acid and paeoniflorin; HPLC content determination of paeoniflorin. [0003] Honeysuckle is the main drug in the prescription. The above-mentioned standards all use TLC of c...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 郭东晓林永强徐丽华崔伟亮
Owner SHANDONG INST FOR FOOD & DRUG CONTROL
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