Rapid detection method of beet syrup characteristics in adulterated honey
A beet syrup and detection method technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to adapt and detect whether honey is mixed with beet syrup, etc., and achieve high selectivity, high sensitivity, and simple operation Effect
Active Publication Date: 2016-06-08
JIANGSU SINOGRAPHY TESTING
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However, after a period of use, these methods can no longer adapt to the needs of the current market environment, such as the inability to detect whether beet syrup is mixed in honey and accurately quantify the proportion of beet syrup mixed
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[0017] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
[0018] see figure 1 , figure 2 , image 3 and Figure 4 , in the embodiment of the present invention, a kind of rapid detection method of beet syrup feature in adulterated honey, the steps are:
[0019] (1) Pretreatment of the method: Accurately weigh 1.0 (±0.01) g of the sample into a 50 ml sample tube, add 10.0 ml of water, vortex and sonicate for 2 min until completely dissolved, and inject the sample after passing through the water phase filter membran...
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The present invention provides a quick determination method of beet syrup characteristic substances in adulterated honey, and the method comprises the following steps: first, pretreatment of the method, to be more specific, accurately weighing a sample of 1.0 (+ 0.01) g to put into a 50ml sample tube, adding 10.0ml water, after volution, completely dissolving the sample by ultrasonic for 2min, filtering with a water phase membrane; then separation by liquid chromatography, to be more specific, phenomenex Kinetx C18100A (100*4.60mm, 2.6 mum) column is selected as a chromatographic column, a mobile phase comprises A methanol and B 0.005% formic acid aqueous solution, gradient elution manner by volume percentage is used; and final mass spectrometric detection, to be more specific, an electrospray ion source (ESI) is used, positive ion MRM scanning is performed under the conditions of the capillary voltage of 3.5KV, the temperature of 350 DEG C, the dry gas (nitrogen) flow of 5L / min, the sheath gas temperature of 400 DEG C and atomizer pressure of 50psig. According to the method, by liquid chromatography tandem mass spectrometry multiple reaction monitoring selection ion analysis of the sample solution, beet syrup in the adulterated honey can be rapidly identified, and the recovery rate is in the range of 95-105%.
Description
technical field [0001] The invention belongs to the technical field of food detection, in particular to a rapid detection method for characteristic substances in beet syrup in adulterated honey. Background technique [0002] Real honey is a natural sweet substance that is fully brewed after bees collect nectar, secretions or honeydew from plants, mix with their own secretions. However, some honey currently on the market uses high-fructose corn syrup, beet syrup, sugarcane syrup, preserved fruit syrup, etc. to mix into honey to make high profits, because it does not destroy the flavor of honey and enhances the detection indicators of glucose and fructose in honey , so it is extremely difficult to distinguish true from false. Although adulterated honey meets the national standard in terms of technical indicators, it does not have all the nutrients of natural honey, which greatly affects the quality of honey and brand reputation, causing confusion in the honey market. The cur...
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IPC IPC(8): G01N30/88
Inventor 徐锦忠刘秀红王英俊王祝超周培培刘花德
Owner JIANGSU SINOGRAPHY TESTING