A method for testing the specificity, consistency and stability of new pure line soybean varieties

Abstract

The invention discloses a method for testing the distinctness, uniformity and stability of a pure-line soybean new variety. The method comprises the following steps: obtaining variation points; determining a testing area of the soybean variety to be tested; establishing a database; after determining the sampling amount, randomly sampling, mixing samples, and extracting DNA in the mixed sample; preparing primers; amplifying the DNA in the mixed sample by using the primers, and establishing a high-throughput sequencing library by using the product generated after amplification; performing high-throughput sequencing on the high-throughput sequencing library, so as to obtain a sequencing fragment group; analyzing the sequencing fragment group, so as to obtain the genotype of the soybean variety to be tested and the genotype of an abnormal plant; obtaining similar varieties, variation points and variation point rate through comparison; after obtaining an abnormal plant variety through comparing the genotype of the abnormal plant and genotypes in the database, computing the abnormal plant rate; judging the distinctness, uniformity and stability of the soybean variety to be tested according to the variation points, variation point rate and abnormal plant rate. The method can be used for accurately and completely judging the distinctness, uniformity and stability of the plant variety to be tested.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for testing the specificity, consistency and stability of a pure soybean new variety. Background technique [0002] As a specialized intellectual property, new plant varieties have become the core competitiveness of a company or even a country. The authorization of new plant varieties and the resolution of related legal issues rely on DUS testing, which is the field planting identification or indoor molecular marker identification of the specificity (Distinctness), uniformity (Uniformity) and stability (Stability) of the tested soybean varieties. The field planting identification process is as follows: plant the soybean variety to be tested and similar varieties in the field at the same time, observe their multiple traits in the growing season of 2 years or more, and judge the significant difference between the soybean variety to be tested and similar varieties based on the ...

AI Technical Summary

Problems solved by technology

[0003] The disadvantages of field planting identification are: long cycle, heavy workload, and environment affecting traits, resulting in inaccurate judgment

The disadvantages of indoor molecular marker identification are: each test area of ​​each sample needs to be processed separately, the workload is heavy, a large number of samples and test areas cannot be sampled, and the rate of hybrid plants cannot be calculated, so stability and consistency tests cannot be performed

The common disadvantage of field planting identification and indoor molecular marker identification is that due to the workload, it is impossible to objectively select similar varieties from existing varieties, which can only be provided by the variety right applicant. Approximate breeds provided by others may not be true, resulting in legal consequences of wrong breed authorization

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