Method for determining specificity, consistency and stability of new product of inbred rape

Abstract

The invention discloses a method for determining specificity, consistency and stability of a new product of inbred rape. The method comprises the following steps: obtaining a variation point; determining a test area of the rape product to be determined; constructing a database; determining sampling amount, performing random sampling and mixing, extracting DNA of the mixed sample; preparing a primer; using the primer for amplifying the DNA of the mixed sample, wherein the amplification products can be used for constructing a high flux sequencing library; performing high flux sequencing on the high flux sequencing library to obtain a sequencing fragment group; analyzing the sequencing fragment group to obtain genotype of the rape product to be detected and abnormal plant genotype; comparing the obtained similar products, variation points and variation site rate; comparing the abnormal plant genotype and genotype in a database, obtaining the abnormal plant products and calculating abnormal plant rate; and determining the specificity, consistency and stability of rape product to be determined by using variation point, the variation site rate and the abnormal plant rate. The method can accurately and completely determine the specificity, consistency and stability of the rape product to be determined, and the test speed is faster.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for testing the specificity, consistency and stability of a new pure-line rape variety. Background technique [0002] As a specialized intellectual property, new plant varieties have become the core competitiveness of a company and even a country. The authorization of new plant varieties and the settlement of related legal issues depend on DUS testing, that is, field planting identification or indoor molecular marker identification of the specificity, uniformity and stability of the rapeseed variety to be tested. The field planting identification process is as follows: plant the rapeseed variety to be tested and the similar variety in the field at the same time, observe their multiple characters in the growing season of 2 years or more, and judge the difference between the rapeseed variety to be tested and the similar variety is significant based on the performance of the tr...

AI Technical Summary

Problems solved by technology

[0003] The disadvantages of field planting identification are: long cycle, heavy workload, and environment affecting traits, resulting in inaccurate judgment

The disadvantages of indoor molecular marker identification are: each test area of ​​each sample needs to be processed separately, the workload is heavy, a large number of samples and test areas cannot be sampled, and the rate of hybrid plants cannot be calculated, so stability and consistency tests cannot be performed

The common disadvantage of field planting identification and indoor molecular marker identification is that due to the workload, it is impossible to objectively select similar varieties from existing varieties, which can only be provided by the variety right applicant. Approximate breeds provided by others may not be true, resulting in legal consequences of wrong breed authorization

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