A method for testing the specificity, consistency and stability of new pure-line rice varieties

Abstract

The invention discloses a method for testing the specificity, consistency and stability of a new pure-line rice variety, which specifically includes: obtaining a variation site; determining the test area of ​​the rice variety to be tested; building a database; Sampling and mixing and extracting the DNA of the mixed sample; preparing primers; using the primers to amplify the DNA of the mixed sample, and the amplified product is used to construct a high-throughput sequencing library; perform high-throughput sequencing on the high-throughput sequencing library to obtain sequencing fragments group; analyze the sequencing fragment group to obtain the genotype of the rice variety to be tested and the genotype of the hybrid; compare and obtain similar varieties, variation sites and variation rate; compare the genotype of the hybrid with the genotype in the database to obtain the genotype of the hybrid After the variety, calculate the hybrid rate; use the variation site, variation rate and hybrid rate to judge the specificity, consistency and stability of the rice variety to be tested. The method can accurately and completely judge the specificity, stability and consistency of the rice variety to be tested.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for testing the specificity, consistency and stability of a new pure line rice variety. Background technique [0002] As a specialized intellectual property, new plant varieties have become the core competitiveness of a company or even a country. The authorization of new plant varieties and the resolution of related legal issues rely on DUS testing, which is the field planting identification or indoor molecular marker identification of the specificity (Distinctness), uniformity (Uniformity) and stability (Stability) of the rice variety to be tested. The field planting identification process is: plant the rice variety to be tested and similar varieties in the field at the same time, observe their multiple traits in the growing season of 2 years or more, and judge the difference between the rice variety to be tested and the similar varieties according to the performance of the...

AI Technical Summary

Problems solved by technology

[0003] The disadvantages of field planting identification are: long cycle, heavy workload, and environment affecting traits, resulting in inaccurate judgment

The disadvantages of indoor molecular marker identification are: each test area of ​​each sample needs to be processed separately, the workload is heavy, a large number of samples and test areas cannot be sampled, and the rate of hybrid plants cannot be calculated, so stability and consistency tests cannot be performed

The common disadvantage of field planting identification and indoor molecular marker identification is that due to the workload, it is impossible to objectively select similar varieties from existing varieties, which can only be provided by the variety right applicant. Approximate breeds provided by others may not be true, resulting in legal consequences of wrong breed authorization

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