A method for determining the specificity, consistency and stability of new hybrid rice varieties

Abstract

The invention discloses a method for measuring the specificity, consistency and stability of a new hybrid rice variety. The method comprises: obtaining variation sites; determining the test area of ​​the rice variety to be tested; constructing a database; after determining the sampling amount, randomly sampling and mixing and extracting the DNA of the mixed sample; preparing primers; using the primers to amplify the DNA of the mixed sample , the amplified product is used to construct a high-throughput sequencing library; perform high-throughput sequencing on the high-throughput sequencing library to obtain a sequenced fragment group; analyze the sequenced fragment group to obtain the genotype of the rice variety to be tested and the hybrid plant genotype; compare the obtained Approximate variety, variation site and variation rate; after obtaining the hybrid variety, calculate the hybrid rate; use the variation site, variation rate and hybrid rate to judge the specificity, consistency and stability of the rice variety to be tested . The method can accurately and completely judge the specificity, stability and consistency of the rice varieties to be tested, and the testing speed is faster.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for measuring the specificity, consistency and stability of a new hybrid rice variety. Background technique [0002] As a specialized intellectual property, new plant varieties have become the core competitiveness of a company or even a country. The authorization of new plant varieties and the resolution of related legal issues rely on DUS testing, which is the field planting identification or indoor molecular marker identification of the specificity (Distinctness), uniformity (Uniformity) and stability (Stability) of the rice variety to be tested. The field planting identification process is: plant the rice variety to be tested and similar varieties in the field at the same time, observe their multiple traits in the growing season of 2 years or more, and judge the difference between the rice variety to be tested and the similar varieties according to the performance of the ...

AI Technical Summary

Problems solved by technology

[0003] The disadvantages of field planting identification are: long cycle, heavy workload, and environment affecting traits, resulting in inaccurate judgment

The disadvantages of indoor molecular marker identification are: each test area of ​​each sample needs to be processed separately, the workload is heavy, a large number of samples and test areas cannot be sampled, and the rate of hybrid plants cannot be calculated, so stability and consistency tests cannot be performed

The common disadvantages of field planting identification and indoor molecular marker identification are: due to the heavy workload, similar varieties cannot be objectively selected from existing varieties, and can only be provided by the applicant for variety rights. However, based on commercial interests and other motives, variety rights Approximate breeds provided by the applicant may not be authentic, resulting in legal consequences of wrong breed authorization

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