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Mongolian medicine long pepper medicine efficacy material basis analysis method

A technology based on material basis and analytical method, applied in the field of traditional Chinese medicine extracts, can solve problems such as toxicity, and achieve the effect of improving extraction rate and simple operation.

Active Publication Date: 2015-07-29
李焕婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research carried out for this purpose proved that piperine in Piperia can inhibit both endogenous and exogenous blood lipid elevation, and at the same time it was learned that piperine may also be toxic.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Purchase 200.00 g of commercially available Mongolian medicinal materials-dried Piperia fruit, carry out ultrafine grinding, pulverize to a particle size of 100-120 μm, put it in a container, then add 2.5 L of ethanol solution with an ethanol mass fraction of 80 wt%, and carry out at 45°C Ultrasonic extraction, the ultrasonic power is 600W, extract for 5 hours, filter to obtain the extract; the extract is adsorbed through a silica gel chromatography column, eluted with water for 3 column volumes, the eluent is discarded, and then methanol-chloroform (methanol and The volume ratio of chloroform is 1: 3.5) as the eluent, eluting, and collecting the eluent; the above-mentioned eluent is concentrated under reduced pressure to obtain a dry extract; the acetone mass fraction of the above-mentioned dry extract is 80wt% Dissolve the acetone solution, put on the AB-8 macroporous adsorption resin column, then use the acetone mass fraction of 3 times column volume as the acetone so...

Embodiment 2

[0049] Purchase 200.00 g of commercially available Mongolian medicinal materials-dried Piperia fruit, carry out ultrafine grinding, pulverize to a particle size of 100-120 μm, put it in a container, then add 2.5 L of ethanol solution with a mass fraction of ethanol of 70 wt%, and carry out at 40°C Ultrasonic extraction, ultrasonic power is 700W, extracts for 4 hours, filters, and obtains the extract; The extract is absorbed through a silica gel chromatography column, washed with water for 3 column volumes, the eluent is discarded, and then methanol-chloroform (volume ratio 1: 3.5) as the eluent, eluted, and collected the eluent; the above-mentioned eluent was concentrated under reduced pressure to obtain a dry extract; the acetone mass fraction of the above-mentioned dry extract was dissolved in an acetone solution , on the AB-8 macroporous adsorption resin column, then eluted with an acetone solution with acetone mass fraction of 3 times the column volume, discarding the eluen...

Embodiment 3

[0052] Purchase 200.00 g of commercially available Mongolian medicinal materials-dry Piperia fruit, carry out ultrafine grinding, pulverize to a particle size of 100-120 μm, put it in a container, then add 2.5 L of ethanol solution with an ethanol mass fraction of 85 wt%, and carry out at 43°C Ultrasonic extraction, ultrasonic power is 600W, extracts for 6 hours, filters, and obtains the extract; The extract is absorbed through a silica gel chromatography column, washed with water for 3 column volumes, the eluent is discarded, and then methanol-chloroform (volume ratio 1: 3.5) as the eluent, eluted, and collected the eluent; the above-mentioned eluent was concentrated under reduced pressure to obtain a dry extract; the above-mentioned dry extract was dissolved in an acetone solution with an acetone mass fraction of 80 wt%. , on the AB-8 macroporous adsorption resin column, then eluted with an acetone solution with acetone mass fraction of 3 times the column volume, discarding t...

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Abstract

The invention relates to a Mongolian medicine long pepper medicine efficacy material basis analysis method. The method comprises the following steps that (i) Mongolian medicine long pepper extracts are prepared, commercially available Mongolian medicine of dried long pepper fruits are purchased and are subjected to ultramicro pulverization, an ethanol solution with the ethanol mass percent being 65 to 100 wt percent is added, ultrasonic extraction is carried out at 40 to 45 DEG C, the time is 4 to 6 hours, the filtering is carried out, and an extracting solution is obtained; (ii) the medicine efficacy material basis of the Mongolian medicine long pepper extracts is subjected to qualitative analysis; an efficient liquid chromatography is used for carrying out qualitative analysis on medicine efficacy material basis ingredients of the Mongolian medicine long pepper extracts, the prepared Mongolian medicine long pepper extracts are dissolved in flowing phases, the separation is carried out through the efficient liquid chromatography, the obtained chromatogram is compared with the chromatogram of the long pepper medicine effect ingredient standard product, and the ingredients of the Mongolian medicine long pepper extracts are analyzed. The method has the advantages that the operation is simple, various medicine effect ingredients of long pepper can be extracted, in addition, the extracting rate of the medicine effect active ingredients is improved, and the method is suitable for industrial production.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a method for analyzing the basis of effective substances of the Mongolian medicinal material Piperumba. Background technique [0002] Mongolian medicine has a long history. It is a summary of the Mongolian people's long-term experience in fighting diseases and absorbing the experience of Tibetan medicine and Han medicine. It is an important part of Chinese national medicine and a medicine with national characteristics. It has been proved to be an indispensable subject in clinical medicine for thousands of years. The Mongolian medicinal material Piperia is the near-ripe or mature fruit ear of the Piperaceae plant, which belongs to the spleen meridian, stomach meridian, large intestine meridian, lung meridian, bladder meridian, liver meridian, and kidney meridian. Indications for cold pain in the epigastric abdomen, vomiting, diarrhea, migraine, nasal sinusitis, external t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 李焕婷薄彧坤杨丹
Owner 李焕婷
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