A method for analyzing the substance basis of the efficacy of Mongolian medicinal material Piperia officinalis

Abstract

The invention relates to a Mongolian medicine long pepper medicine efficacy material basis analysis method. The method comprises the following steps that (i) Mongolian medicine long pepper extracts are prepared, commercially available Mongolian medicine of dried long pepper fruits are purchased and are subjected to ultramicro pulverization, an ethanol solution with the ethanol mass percent being 65 to 100 wt percent is added, ultrasonic extraction is carried out at 40 to 45 DEG C, the time is 4 to 6 hours, the filtering is carried out, and an extracting solution is obtained; (ii) the medicine efficacy material basis of the Mongolian medicine long pepper extracts is subjected to qualitative analysis; an efficient liquid chromatography is used for carrying out qualitative analysis on medicine efficacy material basis ingredients of the Mongolian medicine long pepper extracts, the prepared Mongolian medicine long pepper extracts are dissolved in flowing phases, the separation is carried out through the efficient liquid chromatography, the obtained chromatogram is compared with the chromatogram of the long pepper medicine effect ingredient standard product, and the ingredients of the Mongolian medicine long pepper extracts are analyzed. The method has the advantages that the operation is simple, various medicine effect ingredients of long pepper can be extracted, in addition, the extracting rate of the medicine effect active ingredients is improved, and the method is suitable for industrial production.

Description

technical field

[0001] The invention relates to a traditional Chinese medicine extract, in particular to a method for analyzing the basis of effective substances of the Mongolian medicinal material Piperumba. Background technique

[0002] Mongolian medicine has a long history. It is a summary of the Mongolian people's long-term experience in fighting diseases and absorbing the experience of Tibetan medicine and Han medicine. It is an important part of Chinese national medicine and a medicine with national characteristics. It has been proved to be an indispensable subject in clinical medicine for thousands of years. The Mongolian medicinal material Piperia is the near-ripe or mature fruit ear of the Piperaceae plant, which belongs to the spleen meridian, stomach meridian, large intestine meridian, lung meridian, bladder meridian, liver meridian, and kidney meridian. Indications for cold pain in the epigastric abdomen, vomiting, diarrhea, migraine, nasal sinusitis, external t...