Primers, probes, detection systems and kits for one-time detection of multiple genes in lung cancer

Abstract

The present invention discloses primers, probes, a detection system and a kit for one time detection of lung cancer multiple genes, wherein the primers, the probes, and the distribution way for detecting 25 EGFR gene mutations, 7 KRAS gene mutations, 6 BRAF gene mutations, 9 NRAS gene mutations, 5 HER2 gene mutations, 2 PIK3CA gene mutations, 5 fusion genes of ALK5, 13 fusion genes of ROS1, and 6 fusion genes of RET are provided. According to the present invention, the detection kit adopts the 12 linking PCR reaction strip design, each 12 linking PCR strip detects multiple genes of a sample, and the corresponding fusion detection reagents and the internal control reagents are filled in the pipes 1-4 of the 12 linking PCR strip; and with the primers, the probes, the detection system and the kit, the one-time detection of the 24 fusions and the 54 mutations of the lung cancer can be achieved, such that the detection time is substantially shortened, the sensitivity is high, the specificity is strong, the operation is simple and rapid, and the reference for selection of tumor targeting drug therapy on lung cancer patients can be provided for clinician.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to primers, probes and detection systems for multiple genes of lung cancer. Background technique [0002] Lung cancer is one of the malignant tumors with the highest morbidity and mortality. Among them, non-small cell lung cancer (NSCLC) accounts for about 85% of lung cancers. With the continuous in-depth research on tumor pathogenesis and its biological behavior, more and more people are focusing on molecular targeted therapy characterized by high specificity and low side effects. In recent years, in the field of molecular targeted therapy of lung cancer, research hotspots have mainly focused on EGFR, ALK, ROS1, KRAS, BRAF and other targets. The mutation or fusion status of these genes is related to the efficacy of targeted drugs, and the combination of multiple genes Detection can further improve the accuracy of predicting prognosis. However, at present, most of the mutations ...

AI Technical Summary

Problems solved by technology

However, at present, most of them can only detect one mutation or a mutation of a gene at a time, the detection throughput is low, and they can only be screened one by one, which takes a long time

At the same time, due to the difficulty in obtaining materials for advanced patients and the small sample size, they often cannot meet the needs of screening one by one, and there is no clinical kit that can simultaneously and one-time detect these 9 hotspot genes

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