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Method for manufacturing phenolic acid positions of vernonia anthelmintica and anti-inflammation application of phenolic acid positions

A technology of vernonia phenolic acid and insect repellent vernonia, applied in anti-inflammatory agents, food preparation, medical preparations containing active ingredients, etc.

Inactive Publication Date: 2015-08-12
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been reported that the total extract of Vernonia vernium can inhibit the immune response, but there is no report on the part of Vernonia anthelmintic that has anti-inflammatory activity

Method used

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  • Method for manufacturing phenolic acid positions of vernonia anthelmintica and anti-inflammation application of phenolic acid positions
  • Method for manufacturing phenolic acid positions of vernonia anthelmintica and anti-inflammation application of phenolic acid positions
  • Method for manufacturing phenolic acid positions of vernonia anthelmintica and anti-inflammation application of phenolic acid positions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Preparation of vernophilic acid fraction for repelling insects:

[0021] Grind 10kg of dried Vernonia fruit medicinal materials, add 30 times the volume of aqueous solution, heat and reflux for extraction 3 times at 95°C for 3 hours, cool to room temperature, filter, combine the extracts, and concentrate under reduced pressure until there is no alcohol smell , to obtain alcohol extract;

[0022] Disperse the obtained alcohol extract with 5% ethanol to contain crude drug 100g / L, filter, and then use AB-8 macroporous adsorption resin column to elute with 1 times the column volume of water, and then use 8 times the column volume The concentration is 40% ethanol for elution, the ethanol eluate is collected, concentrated under reduced pressure, and dried in vacuum to form a dry powder, which contains 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and 3, Insect Repelling Vernophilic Acid Fraction of 4-dicaffeoylquinic Acid 390g.

Embodiment 2

[0023] Example 2: Preparation of vernophilic acid fraction for repelling insects:

[0024] Grind 10kg of the dried Vernonia fruit medicinal material, add 3 times the volume concentration of 95% ethanol, heat and reflux for extraction 5 times at room temperature for 3 hours, cool to room temperature, filter, combine the extracts, concentrate under reduced pressure until alcohol-free Taste, to obtain alcohol extract;

[0025] The ethanol extract that obtains is that 5% ethanol is dispersed to contain crude drug 50g / L with concentration, centrifugation (4000 revs / min), DM-130 type macroporous adsorption resin column, first with 3 times column volume water eluting, then use 1 times the column volume concentration is 95% ethanol for elution, collect the ethanol eluate, concentrate under reduced pressure, and dry it in vacuum to dry powder, which contains 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid Anthelmintic Vernophilic Acid Fraction of Acid and 3,4-dicaffeoylquinic ...

Embodiment 3

[0026] Example 3: Preparation of Vernophilic Acid Fraction for Repelling Insects:

[0027] Grind 10 kg of dried Vernonia fruit medicinal materials, add 15 times the volume concentration of 30% ethanol, heat and reflux for extraction 3 times at 75°C for 2 hours, cool to room temperature, combine extracts, and concentrate under reduced pressure until alcohol-free Taste, to obtain alcohol extract;

[0028] The extract that is obtained is that 10% methanol is dispersed to contain crude drug 80g / L with concentration, centrifugation (4000 revs / min), HPD-100 type macroporous adsorption resin column, first with 4 times column volume water elution, then with 6 The column volume concentration is 50% ethanol for elution, the ethanol eluate is collected, concentrated under reduced pressure, and dried in vacuum to dry powder, which contains 3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid's repellent vernophilic acid fraction 440g.

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Abstract

The invention relates to a method for manufacturing phenolic acid positions of vernonia anthelmintica and anti-inflammation application of the phenolic acid positions. The method includes smashing dried fruits of the vernonia anthelmintica; extracting extracts by the aid of organic solvents; separating and purifying the extracts by the aid of resin to obtain the phenolic acid positions of the vernonia anthelmintica. Main active ingredients of phenolic acid at the positions of the vernonia anthelmintica include 3, 4-dicaffeoyl-quinic acid, 3, 5-dicarffeoyl-quinic acid and 4, 5-dicaffeoyl-quinic acid, the total content of the phenolic acid ranges from 20% to 99%, excess release of nitric oxide of inflammation mediators can be obviously suppressed by the phenolic acid positions of the vernonia anthelmintica in inflammation response of mice macrophage RAW264.7 induced by lipopolysaccharide, excessive secretion of interleukin 6, interleukin 1 and tumor necrosis factors alpha of inflammation factors can be suppressed, excess expression of nitric oxide synthase and epoxidase 2 can be suppressed, and phosphorylation of nuclear factors kB can be suppressed. The method has the advantages that the phenolic acid positions of the vernonia anthelmintica has excellent anti-inflammation activity and can be used for preventing or treating inflammation due to the excess nitric oxide, the excess tumor necrosis factors alpha, the excess interleukin 6 or the excess interleukin 1.

Description

technical field [0001] The invention relates to a method for preparing anthelminth-repelling vernonia phenolic acid fraction and its use in anti-inflammatory drugs or health care products, and belongs to the technical field of ethnic medicines. Background technique [0002] Vernonia anthelmintica (L.) Willd, also known as Indian fennel, is an annual tall herb of the family Asteraceae (Asteraceae) of the genus Vernonia. The medicinal part is the mature fruit, which is harvested in autumn and dried. It has three grades of dry heat, and its main effects are to clear abnormal mucus, deworm, reduce swelling, dispel cold and relieve pain. It is the main classic drug for the treatment of vitiligo in traditional Uyghur medicine in the past dynasties. [0003] Vernonia fruit contains sesquiterpene lactones, flavonoids, epoxy octadecenoic acid and its glycerides, triterpenoids, sterols and caffeoylquinic acid and other compounds. It has been reported that the total extract of Vernoni...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P29/00A23L1/29A23L33/00
Inventor 阿吉艾克拜尔·艾萨李浩榕吐鲁洪·卡地尔刘永强信学雷努尔阿米乃·麦麦提
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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