Rat transplantable lung cancer model establishing method

An established method and transplantable technology, which can be used in pharmaceutical formulations, preparations for in vivo experiments, and pharmaceutical sciences. Low rate, easy to grow and transfer, simple to prepare effect
CN104826136AInactive Publication Date: 2015-08-12HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
Publication Date
2015-08-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a rat transplantable lung cancer model establishing method including the following steps: Walker-256 cell ascitic fluid is prepared; Walker-256 cell is inoculated into SD rat (Sprague-Dawley rat) lung; after anesthesia of a SD rat, left part of thoracic cavity of the SD rat is disinfected by use of povidone-iodine, Walker-256 cell suspension and Matrigel in same volume are mixed evenly on the ice to obtain a mixed solution, the mixed solution is slowly injected between 5th rib and 6th rib at left lung into the left lung by use of an injector, the puncture depth is 0.6-0.8 cm, after the injection, a needle is stopped for 10 s, and then is pulled out slowly, and after the rat is grown for 14 days, a transplantable lung cancer model is established. According to the method, the damage to the rat is small, the Walker-256 cell can be directly transplanted into the left lung only by use of a generally anaesthetized rat, formation of tumor lesion part is clear, the tumor lesion is single and controllable in size, and the method also has the advantages of simple preparation, high tumor formation rate, low animal mortality and the like.
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Description

technical field

[0001] The invention relates to the field of constructing experimental animal models of diseases, in particular to a method for establishing rat transplanted lung cancer models. Background technique

[0002] At present, with the aggravation of air pollution in China, especially the raging smog has brought serious hidden dangers to the health of Chinese people. The International Agency for Research on Cancer under the World Health Organization pointed out that there is sufficient evidence to show that smog is still an important factor in inducing lung cancer. Lung cancer has become one of the cancers leading to the highest mortality rate of cancer patients in the world, and is listed as the focus of future cancer prevention and treatment by the Ministry of Health. However, due to the complex clinical manifestations of lung cancer and the difficulty of early detection and diagnosis, the efficacy of lung cancer cannot be improved. Therefore, replicating an ani...

Claims

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