A method for immobilizing hepatitis B antibody on the surface of medical equipment

Abstract

The invention discloses a method for immobilizing hepatitis B antibody on the surface of a medical device, which mainly includes: 1) pretreatment of the surface of the device body, 2) preparation of holes, 3) post-treatment of the surface of the device body, and 4) process steps of fixing the antibody. It is characterized in that: the process steps of immobilizing antibodies are: treating the bracket with holes with citrate buffer solution; immobilizing hepatitis B antibody and incubating; storing at low temperature after drying; providing serum albumin nutrient buffer solution and incubating; cyclically variable temperature incubation ; fluorescently labeled and incubated; washed and allowed to dry. Compared with the prior art, the present invention has the advantages of high firmness of antibody immobilization, high activity of the antibody on the scaffold, and high detection accuracy.

Description

Technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a method for fixing hepatitis B antibody on the surface of medical equipment. Background technique [0002] Biochip technology fixes proteins, cells, tissues, growth factors, antibodies, etc. on the surface of supports or medical devices, and then hybridizes with fluorescently labeled DNA sample molecules, and obtains samples by detecting the hybridization signal intensity of each probe molecule The number and sequence information of molecules is one of the most promising DNA analysis techniques. Hepatitis B antibody is a protective or neutralizing antibody produced after hepatitis B virus surface antigen (HBsAg) stimulates the human immune system. It can neutralize the infectivity of hepatitis B virus and protect the human body from re-attacks by hepatitis B virus. At present, the most effective way to prevent hepatitis B is to inject hepatitis B vaccine to mak...

AI Technical Summary

Problems solved by technology

However, this method is to first fix the antibody on the support, and then go through the washing step, and use a phosphate buffer solution that is harsh on the pH value, which has the following defects: 1) the support is not pretreated (without phosphate buffer solution soaking or spraying), immersing the scaffold in the phosphate buffer solution of the antibody will undoubtedly dilute the concentration of the phosphate buffer solution and change the original pH value, which is unfavorable for the incubation of the antibody; If the scaffold is washed with phosphate buffer solution, the attached antibody will be detached, thus reducing the amount of antibody fixed on the scaffold; 3) the phosphate buffer solution loses its buffering capacity when the pH value is above 7.5, and it will interact with heavy metal ions The reaction occurs and precipitates in the form of phosphate, which will also affect the detection