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Identification method of harpagoside in ginseng superfine powder

A technology of harpagoside and ultrafine powder, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of difficulty in reaching, waste of reagents and medicinal materials, and inability to reduce costs, and achieves clear identification spots, accurate identification results, and easy results. Effect

Inactive Publication Date: 2017-03-15
HENAN KANGXING PHARMA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The Chinese Veterinary Pharmacopoeia 2010 edition has a detailed description of the thin-layer identification method for the medicinal material Scrophulariae Scrophulariae. However, when using this method to identify harpagosides for compound preparations containing Scrophulariae Scrophulariae, the spots are not clear, and it is difficult to meet the requirements of the Chinese Veterinary Pharmacopoeia.
Moreover, although the TLC identification of Scrophulariaceae in the prior art has been mentioned, because the active ingredient extraction is not complete enough when preparing the test solution, reagents and medicinal materials are wasted, and the cost cannot be reduced.

Method used

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  • Identification method of harpagoside in ginseng superfine powder
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  • Identification method of harpagoside in ginseng superfine powder

Examples

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Effect test

Embodiment 1

[0034] A thin-layer chromatography identification method for detecting harpagoside in ginseng superfine powder, characterized in that the test method comprises the following steps:

[0035] The first step is to take ginseng medicinal materials, wash them once with ethanol, then wash them twice with deionized water, and dry them; then use a pulverizer to coarsely grind them, mix them and dry them at 60-80°C until the water mass percentage is at 6% or less, then use a two-way airflow ultrafine powder sieve machine to perform ultrafine pulverization at room temperature, collect the medicines sieved through a 250-300 mesh screen, and then mix them to obtain superfine ginseng grass powder;

[0036] The second step, preparation of the test solution: take an appropriate amount of ginseng superfine powder, add methanol, and prepare 1 mL of methanol containing 1.0-1.2 g of ginseng superfine powder, soak for 1-2 hours; The power is 250W, the frequency is 33kHz, the time is 30-60 minutes...

Embodiment 2

[0041] A thin-layer chromatography identification method for detecting harpagoside in ginseng superfine powder, characterized in that the test method comprises the following steps:

[0042] The first step is to take the ginseng medicinal materials, wash them once with ethanol, then wash them twice with deionized water, and dry them; then use a pulverizer to coarsely grind them, mix them and dry them at 70-75°C until the water mass percentage is at 6% or less, then use a two-way airflow ultrafine powder sieve machine to perform ultrafine pulverization at room temperature, collect the medicines sieved through a 280-300 mesh screen, and then mix them to obtain superfine ginseng grass powder;

[0043] The second step, preparation of the test solution: take an appropriate amount of ginseng superfine powder, add methanol, and prepare 1 mL of methanol containing 1.0-1.2 g of ginseng superfine powder, soak for 1-2 hours; The power is 250W, the frequency is 33kHz, the time is 30-60 min...

Embodiment 3

[0048] A thin-layer chromatography identification method for detecting harpagoside in ginseng superfine powder, characterized in that the test method comprises the following steps:

[0049] The first step is to take ginseng herbs, wash them once with ethanol, then wash them twice with deionized water, and dry them; then use a pulverizer to coarsely grind them, mix them and dry them at 80°C until the water mass percentage is 6% Next, use a two-way airflow ultrafine powder sieve machine to perform ultrafine pulverization at normal temperature, collect the medicine sieved through a 300-mesh sieve, and then mix to obtain superfine ginseng grass powder;

[0050] The second step, preparation of the test solution: take an appropriate amount of ginseng superfine powder, add methanol, and prepare 1 mL of methanol containing 1.0-1.2 g of ginseng superfine powder, soak for 1-2 hours; The power is 250W, the frequency is 33kHz, the time is 30-60 minutes, let cool, and filter; evaporate the...

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Abstract

The invention discloses a method for indentifying harpagoside in ginseng ultrafine powder. The provided method can be used to qualitatively analyze harpagoside in ginseng ultrafine powder, is developed on the basis of common thin-layer chromatography on traditional herbal namely radix scrophulariae, and can be used to identify the components of compound veterinary drugs. The method can be used to identify the negative control, which does not contain any radix scrophulariae, and the results show that the spot is clear and the result is ideal when the identification method is used to identify compound ginseng ultrafine powder in veterinary drugs; the thin-layer chromatography detection way is largely enlarged, and the thin-layer chromatography revised and enlarged rate of preparation in veterinary drugs is effectively improved.

Description

technical field [0001] The invention relates to a thin-layer identification method for superfine powder components, and relates to a test method capable of qualitatively determining harpagoside in compound Chinese medicine preparations. In particular, it relates to a related technology of sample processing and detection for determining harpagoside in ginseng superfine powder by thin-layer chromatography. Background technique [0002] At present, in the quality standards of various Chinese veterinary drug preparations in the country, the TLC identification of its component drugs is about 30% of the total amount of prescription Chinese medicinal materials on average, and the content determination ratio of its component drugs is relatively small, so it is impossible to monitor the quality of preparations in an all-round way. Especially for ultra-fine powder preparations, the microscopic characteristics of medicinal materials no longer exist, and microscopic identification is he...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
Inventor 马霞周延州郭振环王兴涛刘永录杨帅
Owner HENAN KANGXING PHARMA
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