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Composition of specifically excitable GABAergic neurons in nucleus accumbens and its application in improving abnormal behavior in schizophrenia

A schizophrenia and neuron technology, applied in the field of GABA neuron composition, can solve the problem of not providing a method for abnormal behavior of schizophrenia, so as to improve the ability to avoid danger, improve the ability to avoid related unfavorable environments, achieve specific effects

Active Publication Date: 2018-03-27
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether the nucleus accumbens can be used as a target to regulate abnormal behaviors in schizophrenia has not been studied, and the existing technology has not yet provided a method for specifically intervening in the nucleus accumbens to improve abnormal behaviors in schizophrenia

Method used

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  • Composition of specifically excitable GABAergic neurons in nucleus accumbens and its application in improving abnormal behavior in schizophrenia
  • Composition of specifically excitable GABAergic neurons in nucleus accumbens and its application in improving abnormal behavior in schizophrenia
  • Composition of specifically excitable GABAergic neurons in nucleus accumbens and its application in improving abnormal behavior in schizophrenia

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Experimental program
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Embodiment 1

[0033] (1) Preparation of a lentiviral vector carrying the Syn-ChR2-eYFP plasmid.

[0034] Prepare a lentiviral vector for infecting the nucleus accumbens in living animals, which carries a Syn-ChR2-eYFP plasmid: the plasmid (0.5 μg) (the structure of the fusion plasmid is as follows Figure 1A shown), together with the mixed plasmids pCMVΔR8.74 and pMD2.G. (1.5 μg) related to lentiviral packaging, were transfected into 293FT cells (products of ATCC Company) using liposomes (products of Invitrogen Company), the cells were passaged After 25 passages, a new batch of cell lines needs to be replaced) (6 μL liposomes per 200,000 cells). After 24 hours, the culture medium for culturing 293FT cells was replaced with serum-free DMEM containing 5 mM sodium pyruvate and continued incubation. After 16 hours, the ultracentrifuge was used to rupture the membrane at a speed of 50,000 g, the suspension was passed through a 20% sucrose filter column, and the supernatant was collected. The v...

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Abstract

Provided are a composition for specifically exciting GABAergic neurons in the nucleus accumbens and the use thereof in improving abnormal schizophrenic behaviours. The composition comprises a viral vector carrying a light-sensitive gene for infecting GABAergic neurons in the nucleus accumbens and an irradiation device capable of generating blue light, wherein the viral vector comprises a Syn promoter, a light sensation gene and a green fluorescence labelling gene.

Description

technical field [0001] The invention relates to a composition for specifically exciting GABAergic neurons in the nucleus accumbens and its application in improving abnormal behaviors of schizophrenia. Background technique [0002] The epidemiological survey of mental illness in 12 regions in China shows that there are currently about 100 million people in the country suffering from mental illness, and about 16 million people with severe mental illness, and about 250,000 people die by suicide every year. Among the 340 million children and adolescents under the age of 17 in China, about 30 million suffer from emotional disorders and psychological and behavioral problems. Neuropsychiatric diseases currently rank first in the total burden of disease in my country, accounting for one-fifth of all diseases and trauma-induced disabilities and labor losses. According to experts' prediction, the 21st century will be the century of the prevalence of mental illnesses. With the acceler...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/867
CPCA61N5/06C12N15/867
Inventor 屠洁王立平杨帆刘运辉
Owner SHENZHEN INST OF ADVANCED TECH
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