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Composition for specifically stimulating astrocytes and its application in improving abnormal behaviors of schizophrenia

An astrocytes, schizophrenia technology, applied in the application, drug combination, plant genetic improvement and other directions, can solve the lack of cell specificity, the cell mechanism of abnormal behavior of schizophrenia is unclear, and the treatment effect of schizophrenia is not clear. Significant problems such as achieving specificity and improving the effect of abnormal behavior in schizophrenia

Active Publication Date: 2017-12-26
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Deep Brain Stimulation (DBS), which has emerged in recent years, is to electrically stimulate a certain brain region, and it has also achieved certain clinical effects. The cellular mechanism is unclear
Whether it is traditional drug therapy or DBS intervention, due to the lack of cell specificity, it is impossible to achieve real targeted intervention, and it is only a "palliative".
At present, the treatment effect of schizophrenia is not significant, the prognosis is poor, and it is impossible to improve the abnormal behavior of schizophrenia in a targeted manner

Method used

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  • Composition for specifically stimulating astrocytes and its application in improving abnormal behaviors of schizophrenia
  • Composition for specifically stimulating astrocytes and its application in improving abnormal behaviors of schizophrenia
  • Composition for specifically stimulating astrocytes and its application in improving abnormal behaviors of schizophrenia

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Experimental program
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Embodiment 1

[0029] (1) Preparation of lentiviral vector carrying GFAP-ChR2-eYFP plasmid.

[0030] Prepare a lentiviral vector for infecting astrocytes in living animals, which carries a GFAP-ChR2-eYFP plasmid: a vector containing a light-sensitive gene, a green fluorescent marker gene, and a promoter GFAP for specifically labeling glial cells Plasmid (0.5μg) (the fusion plasmid structure is as follows figure 1 shown in A), together with the mixed plasmids pCMVΔR8.74 and pMD2.G. (1.5 μg) related to lentiviral packaging, were transfected into 293FT cells (products of ATCC Company, the After the cells have been subcultured to 25 passages, a new batch of cell lines needs to be replaced) (6 μL liposomes per 200,000 cells), and the specific transfection operation uses the calcium phosphate mammalian transfection kit (Promega, E1200) according to its instructions The description proceeds. After 24 hours, the culture medium for culturing 293FT cells was replaced with serum-free DMEM containing ...

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Abstract

The invention provides a composition for specifically exciting astrocytes and its application in improving abnormal behavior in schizophrenia, the composition comprising: astrocytes for infecting basolateral amygdala astrocytes of the brain A viral vector carrying a light-sensing gene; the vector includes a promoter, a light-sensing gene, and a green fluorescent marker gene, wherein the promoter is a GFAP promoter; an illumination device capable of producing blue light is used for the infection of star-shaped Glial cells perform light regulation to excite them. The composition of the invention can precisely and specifically excite the astrocytes of the basolateral amygdala of the brain, and can effectively improve the abnormal behavior of schizophrenia.

Description

technical field [0001] The invention relates to a composition for specifically stimulating astrocytes and its application in improving abnormal behaviors of schizophrenia. Background technique [0002] Schizophrenia and many other mental diseases have always been the main diseases that threaten human health and disturb the normal life of human beings. According to the epidemiological survey of psychiatric diseases in 12 regions in China, there are currently about 16 million patients with severe mental diseases in the country. Moderate and mild patients, the total can be as high as 67 million. Mental illness ranks first in my country's total disease burden, accounting for 20%, and it is expected that by 2020, this figure will continue to rise to 25%. Therefore, for many years, many neuroscientists around the world have been trying many new therapeutic targets and methods, but there is still no cure for it. Therefore, research on the etiology and new therapeutic targets of s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/867C12N13/00A61K48/00A61K41/00A61P25/18
Inventor 屠洁王立平杨帆刘运辉
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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