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PLA2R antibody quantitative detection test strip and manufacturing and detection methods

A PLA2R, quantitative detection technology, used in measurement devices, instruments, disease diagnosis, etc., can solve the problems of inability to quantify the stability of colloidal gold, easy shedding of molecules, and instability, and achieve short detection time, high sensitivity, and high stability. Effect

Inactive Publication Date: 2015-08-19
SHENZHEN BLOT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the colloidal gold label is based on the principle of electrostatic adsorption, it is unstable in the fluid, and the labeled molecules are easy to fall off, and can only be read when the colloidal gold particles are enriched to a certain amount visible to the naked eye. Qualitative analysis of the detected object
Currently, rapid detection of phospholipase A2 receptor (PLA2R) antibodies by colloidal gold immunochromatography is only mentioned in the Chinese patent application with publication number CN102159951A, but as mentioned above, it cannot be quantified and colloidal gold has poor stability

Method used

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  • PLA2R antibody quantitative detection test strip and manufacturing and detection methods
  • PLA2R antibody quantitative detection test strip and manufacturing and detection methods
  • PLA2R antibody quantitative detection test strip and manufacturing and detection methods

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A method for making a PLA2R antibody detection test strip, comprising the following steps:

[0037] a. Conjugate the mouse anti-human IgG4 antibody with the quantum dot to obtain the quantum dot-mouse anti-human IgG4 antibody complex, and spray it on the conjugate pad;

[0038] b. Coat PLA2R onto nitrocellulose membrane as a detection line, and coat anti-antibody on nitrocellulose membrane as a quality control line, and the distance between the detection line and the quality control line is 5 mm.

[0039] c. Lap the sample pad, the conjugate pad sprayed with the quantum dot-mouse anti-human IgG4 antibody complex, the nitrocellulose membrane with the detection line and the quality control line, and absorbent paper on the sticky bottom plate in sequence. The test strips overlapping each other by 1mm, glued and cut into a width of 4mm are PLA2R antibody detection test strips.

[0040] Method for preparing quantum dot-mouse anti-human IgG4 antibody complexes by coupling an...

Embodiment 2

[0072] A method for making a PLA2R antibody detection test strip, comprising the following steps:

[0073] a. Conjugate the mouse anti-human IgG4 antibody with the quantum dot to obtain the quantum dot-mouse anti-human IgG4 antibody complex, and spray it on the conjugate pad;

[0074] b. Coat PLA2R on the nitrocellulose membrane as the detection line, and coat the anti-antibody on the nitrocellulose membrane as the quality control line. The distance between the detection line and the quality control line is 3mm.

[0075] c. Lap the sample pad, the conjugate pad sprayed with the quantum dot-mouse anti-human IgG4 antibody complex, the nitrocellulose membrane with the detection line and the quality control line, and absorbent paper on the sticky bottom plate in sequence. The test strips overlapping each other by 1mm, glued and cut into a width of 4mm are PLA2R antibody detection test strips.

[0076] Method for preparing quantum dot-mouse anti-human IgG4 antibody complexes by co...

Embodiment 3

[0104] A method for making a PLA2R antibody detection test strip, comprising the following steps:

[0105] a. Conjugate the mouse anti-human IgG4 antibody with the quantum dot to obtain the quantum dot-mouse anti-human IgG4 antibody complex, and spray it on the conjugate pad;

[0106] b. Coat PLA2R onto the nitrocellulose membrane as the detection line, and coat the anti-antibody on the nitrocellulose membrane as the quality control line. The distance between the detection line and the quality control line is 10mm.

[0107] c. Lap the sample pad, the conjugate pad sprayed with the quantum dot-mouse anti-human IgG4 antibody complex, the nitrocellulose membrane with the detection line and the quality control line, and absorbent paper on the sticky bottom plate in sequence. The test strips overlapping each other by 1mm, glued and cut into a width of 4mm are PLA2R antibody detection test strips.

[0108] Method for preparing quantum dot-mouse anti-human IgG4 antibody complexes by...

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Abstract

The invention relates to the technical field of biology, in particular to a PLA2R antibody quantitative detection test strip and manufacturing and detection methods. The test strip uses a principle of detecting an antibody with an indirect method and comprises the steps of coating PLA2R on a nitrocellulose membrane respectively; spraying a quantum dot-coupled anti-human IgG antibody on a conjugate mat, then overlapping a sample mat, the conjugate mat, the nitrocellulose membrane and water absorption paper on an adhesive PVC bottom plate in sequence, cutting into test strips of certain width and assembling into a detection card, wherein an antibody concentration value can be detected by a fluorescence analyzer, and is used for quantitatively detecting the PLA2R antibody in the sample. By using a quantum dot immunochromatographic method, the content of the PLA2R antibody in serum, plasma and whole blood can be safely, precisely and fast detected in a non-invasive, low-risk and low-price manner, and an auxiliary action can be provided for preliminary screening and illness monitoring of idiopathic membranous nephropathy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a PLA2R antibody quantitative detection test strip and a production and detection method. Background technique [0002] According to the etiology, membranous nephropathy can be divided into idiopathic and secondary membranous nephropathy, and its diagnosis depends on clinical manifestations and nephrocentesis. Nephrocentesis is an invasive diagnostic method that does some harm to the patient. Foreign studies have found that about 75% of patients with idiopathic membranous nephropathy can detect anti-PLA2R antibodies through serological testing. Domestic literature also shows that the positive rate of detection in Chinese people is higher, reaching 82%, while secondary membranous nephropathy and The detection rate of patients with other types of glomerular diseases is very low, and the detection rate of healthy people is negative. Quantitative detection of serological PLA2R antibody...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N21/64
CPCG01N33/588G01N21/6402G01N33/558G01N2800/347
Inventor 张永顶马伟民马新民张大准
Owner SHENZHEN BLOT BIOTECH
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