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Application of centella selected triterpenes to preparing medicines for preventing or treating senile dementia

A kind of centella asiatica total glycosides, senile dementia technology, applied in the field of medicine

Inactive Publication Date: 2015-08-26
上海维京生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no report about its use in various medicines or health food or food for the prevention and treatment of senile dementia

Method used

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  • Application of centella selected triterpenes to preparing medicines for preventing or treating senile dementia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] I. Preparation process

[0014] Take 10 kg of Centella asiatica and crush it, pass through a 20-mesh sieve, add 5 times the amount of 70% ethanol to extract twice at room temperature, each time for 1 hour, extract the extract under reduced pressure and recover ethanol to obtain extract, the extract is mixed with water, and then used The same amount of water-saturated n-butanol was extracted twice, the extract was decompressed and the solvent was recovered to obtain a paste, dissolved in a small amount of methanol, and several times the amount of anhydrous acetone was added to precipitate the precipitate, and the precipitate was vacuum-dried.

[0015] II. Asiatica total glycosides content determination (high performance liquid chromatography)

[0016] Chromatographic conditions and system suitability test Octadesilane-bonded silica gel was used as filler, acetonitrile:methanol:water (22:28:50) was used as mobile phase, detection wavelength was 204nm, and flow rate was 0....

Embodiment 2

[0020] I. Preparation process

[0021] Take 10 g of the product prepared in Example 1, add 100 ml of water to dissolve, adjust the pH value to 2-3, extract 3 times with an equal volume of chloroform, take the water layer, adjust the pH value to 6-7, and extract it with an equal volume of n-butanol respectively 3 times, the extract was decompressed and the solvent was recovered to obtain a paste, which was dissolved with a small amount of 95% ethanol and dried in vacuum to obtain the refined product.

[0022] II. Asiatica total glycosides content determination (high performance liquid chromatography)

[0023] Chromatographic conditions and system suitability test Octadesilane-bonded silica gel was used as filler, acetonitrile:methanol:water (22:28:50) was used as mobile phase, detection wavelength was 204nm, and flow rate was 0.8ml / min.

[0024] Determination method Accurately weigh the appropriate amount of reference substances of madecassoside and madecassoside, and use meth...

Embodiment 3

[0026] Embodiment 3 animal test

[0027] I. Experimental drugs

[0028] Centella selected triterpenes (CST), with a content of more than 20% (obtained in Examples 1 and 2), is dissolved in artificial cerebrospinal fluid before use.

[0029] Streptozotocin (STZ), a product of Sigman, was dissolved in artificial cerebrospinal fluid before use.

[0030] Artificial cerebrospinal fluid (ACSF, mmol / L), ACSF composition: NaCl147, KCl2.9, MgCl 2 1.6, CaCl 2 1.7, dextrose 2.2.

[0031] II. Experimental animals

[0032] Inbred C57BL / 6 healthy male Wistar rats, 3-4 months old, 200-250 g, were provided by Shanghai Xipuer-Bikay Laboratory Animal Co., Ltd. Rats were fed under normal conditions, that is, the temperature of the feeding room was 18-20°C, light and dark were alternated for 12 hours each, and they could eat and drink freely. Animals were divided into: normal group, control group, CST low-dose group, CST high-dose group, 15 rats in each group.

[0033] III. Experimental me...

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Abstract

The invention relates to application of centella selected triterpenes (CST) to preparing medicines or health foods or foods for preventing or treating senile dementia. The centella selected triterpenes refers to a compound made of a mixture or a single ingredient which is extracted from Centella asiatic L.Urban., and the content of triterpenoid (asiaticoside, madecassoside, asiatic acid, madasiatic acid and the like) is higher than 20%. Animal experiment results indicate that the centella selected triterpenes can obviously improve hypomnesis, the cognitive function and the behavior disorder of injection beta-amyloid protein (Abata-amyloid protein) molding animals or ventricle injection streptozotocin molding animals, and it is indicated that the centella selected triterpenes have the important application value on preventing and treating the senile dementia in clinic.

Description

technical field [0001] The present invention relates to the field of medicine. It specifically relates to the application of asiaticosides in the preparation of medicines for preventing or treating senile dementia or health food or food. Background technique [0002] With the acceleration of population aging process, senile diseases have become a prominent problem that obviously affects human health. Alzheimer's disease is a common morbid reaction after people enter old age. Its main manifestations are the rapid decline of memory function and the impairment of recognition ability. It is a progressive neurodegenerative disorder. According to reports at home and abroad, with age, under the influence of human tissue aging and genes, the incidence of dementia continues to increase. Senile dementia, malignant tumors, and cardiovascular and cerebrovascular accidents are listed as the leading causes of death in the elderly. The three major diseases are known as the fourth enemy o...

Claims

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Application Information

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IPC IPC(8): A61K36/23A23L1/29A61P25/28A23L33/00
Inventor 栾德刚董伦栾松平肖智俊
Owner 上海维京生物医药科技有限公司
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