Biosensor and method for manufacturing same
A biosensor and electrode technology, which is applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve problems such as fluctuations, problems in the measurement accuracy of glucose concentration in blood, and inability to deal with hematocrit values, etc., and achieve good accuracy.
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experiment example 1
[0107] Experimental example 1. Study on AWP concentration
[0108] [method]
[0109] On the gold electrode 104 fabricated using a printing mask formed by screen printing:
[0110] (1) A water-soluble photosensitive resin composition comprising a compound in which azide-based photosensitive groups are suspended from polyvinyl alcohol and a saponified polyvinyl acetate (manufactured by Toyosei Co., Ltd., product name: BIOSURFINE-AWP, hereinafter called AWP) 0.5% aqueous solution, 1ml;
[0111] (2) AWP 1% aqueous solution, 1ml;
[0112] (3) AWP 2% aqueous solution, 1ml,
[0113] Dry at 37°C for 45 minutes at 60mJ / cm 3 UV (352nm) irradiation (30 seconds under CHIBI LIGHT model-1), put it in a box with silica gel, and store it at room temperature. Potassium ferricyanide 100mM, glucose dehydrogenase (hereinafter referred to as GDH) 2unit / ml, 100mM potassium phosphate buffer (hereinafter referred to as PPB) (pH7.5), washed various hematocrit values (hereinafter referred to as ...
experiment example 2
[0117] [method]
[0118] Apply 1 ml of AWP 1% aqueous solution on the gold electrode 104 produced using a printing mask formed by screen printing, and dry at 37° C. for 45 minutes at 60 mJ / cm 3 UV (352nm) irradiation (30 seconds under CHIBI LIGHT model-1), put it in a box with silica gel, and store it at room temperature. Add potassium ferricyanide 100mM, GDH 1unit / ml, 100mM PPB (pH7.5), washed horse erythrocytes Ht0, 20, 40, 55 + glucose 20, 100, 400, 800mg / dL, apply closed circuit 0mV After 5 seconds, a closed circuit +200 mV was applied at each sampling time to measure the current value.
[0119] [result]
[0120] Figure 7 (a)~(d), Figure 8 (a)~(d) and Figure 9 (a) to (d) show graphs plotting the current values at the downsampling times of 1, 5, and 20 seconds for each hematocrit value when Ht40 is 100%. There was an effect of AWP at any concentration of glucose, and the effect of hematocrit was smaller compared to those without AWP.
experiment example 3
[0122] [method]
[0123] 1. Coating on the gold electrode 104 produced using a printing mask formed by screen printing: (4) AWP 0.5% aqueous solution + glucose dehydrogenase (hereinafter referred to as GDH) becomes 2 unit / The amount in ml, 1ml; (5) AWP 1% aqueous solution + GDH condenses to 0.8ml and becomes 2unit / ml, 1ml; (6) AWP 2% aqueous solution + GDH becomes 2unit / ml when condensed to 0.8ml Quantity, 1ml, dry at 37°C for 45 minutes, carry out 60mJ / cm 3 UV (352nm) irradiation (30 seconds under CHIBI LIGHT model-1), put it in a box with silica gel, and store it at room temperature. Mix potassium ferricyanide 100mM, GDH2unit / ml, 100mM PPB (pH7.5), washed horse erythrocytes Ht0, Ht20, Ht40, and 100mg / dL glucose solution (for the sensor loaded on the electrode, GDH has been removed solution) was added to the gold electrodes (4) to (6) or gold electrodes that were not loaded, and a closed circuit of 0mV was applied for 5 seconds, and a closed circuit of +200mV was applied f...
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