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Traditional Chinese medicinal effective ingredient composition and application thereof to preparation of anti-tumor metastasis drugs

An active ingredient and tumor metastasis technology, applied in the field of medicine, can solve the problems of unclear mechanism, restricting traditional Chinese medicine, and inability to evaluate clinical efficacy, and achieve the effect of reducing the level of phosphorylation

Inactive Publication Date: 2015-09-30
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to many deficiencies and limitations of traditional Chinese medicine and compound prescriptions, such as unknown ingredients, unclear mechanism, difficult quality control, and inability to evaluate clinical efficacy, it restricts the role of traditional Chinese medicine in tumor treatment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Cell Migration Scratch Test A549 cells cultured to the logarithmic phase were taken, digested with 0.25% trypsin, blown into a single cell suspension with a dropper, adjusted the cell density to 1×105 cells / mL, and inoculated in 6 1 mL per well on a well plate was placed in a cell culture incubator at 37°C, 5% CO2 and saturated humidity for 24 hours until the cells were basically confluent. Use a 100 μL micropipette tip to scratch vertically in the 6-well plate, rinse twice with PBS solution, and then add component compatibility solution, 1 mL per well, and add an equal volume of cell culture solution to the negative control group. Place the culture plate in a 37°C, 5% CO2 incubator for 24h and 48h, observe and take pictures with an inverted phase-contrast microscope, respectively, and use Image J software to randomly select 3 scratches and calculate the distance of the scratch blank area in the 100× field of view.

[0028] Results: See the appendix for the r...

Embodiment 2

[0029] Example 2: Cell Migration Experiment of High-Content Cell Imaging System The suspension of blue fluorescent microspheres was put into a 96-well plate, and incubated at 37° C. in the dark for 1 hour. Wash 5 times with 200 μL 1×Wash Buffer, digest and suspend the cells in serum-free culture medium, pipette evenly to adjust the cell density to 1×104 cells / mL, inoculate 50 μL cell suspension per well, and place at 37°C. After culturing in a cell incubator for 24 hours under 5% CO2 and saturated humidity conditions, administer the drug, add an equal volume of cell culture fluid to the negative control group, and add the group-combined test solution for the drug, 100 μL per hole, and set 3 replicates in each group. hole. Place the culture plate in a 37°C, 5% CO2 incubator for 48 hours. Take the 96-well plate that has been cultured for 48 hours, discard the cell culture supernatant, add 200 μL of 5.5% preheated fixative to each well, incubate at room temperature for 60 minute...

Embodiment 3

[0031] Example 3: Transwell experiment of real-time cell sensing resistance instrument A layer of Matrigel gel was added to the chamber of the CIM-plate cell migration and infiltration detection plate, and 600 μL of culture solution containing fetal bovine serum was added to the lower chamber. Then add cell suspension to the upper chamber, adjust the cell density to 1×106 cells / mL, 200 μL per well, and culture in a cell incubator at 37°C, 5% CO2 and saturated humidity for 24 hours. A group matching group and a negative control group were set up, and each group had 3 duplicate wells, which were dynamically detected by a cell sensing resistance instrument for 48 hours.

[0032] Results: The results of the combination of components on the invasion of lung cancer A549 cells are shown in Figure 5 and 6 As shown, compared with the negative control group, the combination of the groups significantly reduced the number of cell invasion (P<0.01), suggesting that the combination of the...

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PUM

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Abstract

The invention discloses a traditional Chinese medicinal effective ingredient composition and application thereof to preparation of anti-tumor metastasis drugs. The traditional Chinese medicinal effective ingredient composition comprises effective dosage of total salvianolic acids, total ginsenoside and ginseng polysaccharides, as well as pharmaceutically acceptable carriers. The important point of the invention is the discovery that the composition can effectively inhibit tumor cell invasion and migration, and significantly reduce the tumor cell ERK1 / 2 phosphorylation level; a preparation prepared according to the matching ratio range provided by the invention has a better curative effect, and overcomes the defects that the traditional drug matching is unclear in active ingredients, the quality is uncontrollable, and accordingly the treating effect is undefined. Therefore, the traditional Chinese medicinal effective ingredient composition has application prospects to the preparation of anti-tumor metastasis drugs.

Description

technical field [0001] The invention belongs to the field of medicine, in particular, the invention relates to a composition of active ingredients of traditional Chinese medicine and its application in the preparation of anti-tumor metastasis medicine. Background technique [0002] Malignant tumors are currently an important disease that endangers human health. Since the 1970s, the incidence and mortality of malignant tumors in my country have been on the rise. Metastasis is a characteristic manifestation of the malignant biological behavior of tumors, and it is also the main reason for the failure of malignant tumor treatment and the death of patients. Therefore, anti-tumor metastasis is the focus of comprehensive tumor therapy. [0003] Tumor metastasis is a multi-step, multi-pathway, and a series of complicated processes involving multi-gene changes. Among them, abnormal activation of the mitogen-activated protein kinase (MAPK) family is associated with tumor metastasis,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61P35/04A61K31/715
Inventor 毕蕾陈卫平杨烨
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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