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Column temperature controlling high borosilicate glass interlayer chromatography column

A silicate glass and chromatographic column technology, applied in chemical instruments and methods, separation methods, solid adsorbent liquid separation, etc., to achieve the effect of high strength, large temperature change, and improved separation efficiency

Inactive Publication Date: 2015-09-30
ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN200910089340.5 discloses a constant-pressure sealed upward chromatographic column device, Chinese patent CN200920316761.2 discloses an automatic liquid-adding chromatographic column, and CN201420166784.0 discloses a glass containing a liquid reservoir and a column Chromatographic column, the TLC Mesh chromatographic column developed by Li Yulin of Fuzhou University based on the basic principles of high performance liquid chromatography is the same as the common glass chromatographic column, and the device can only achieve normal temperature separation operation

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Embodiment 1

[0022] A high borosilicate glass interlayer chromatography column with controllable column temperature of the present invention can be applied to the separation process of licorice flavonoids and triterpene saponins in licorice paste by pH gradient elution method, which can effectively control the column layer Column temperature during analysis. The specific implementation steps are as follows:

[0023] 1. Preparation of loading solution

[0024] The licorice fluid extract to be recycled after extracting polysaccharides was stirred and dissolved with purified water at room temperature (fluid extract:purified water volume ratio 1:4), centrifuged for 20min (speed 4000r / min), and the supernatant was prepared into a suitable concentration as the loading solution.

[0025] 2. Pretreatment of macroporous resin

[0026] Soak the pharmaceutical-grade resin HPD400, which was optimized by the static adsorption and elution experiment, in ethanol for 24 hours, then wash the resin with ...

Embodiment 2

[0031] A high borosilicate glass interlayer chromatography column with controllable column temperature of the present invention can be applied to the separation process of licorice flavonoids and triterpene saponins in licorice paste by pH gradient elution method, which can effectively control the column layer Column temperature during analysis. The specific implementation steps are as follows:

[0032] 1. Preparation of loading solution

[0033] The licorice fluid extract to be recycled after extracting polysaccharides was stirred and dissolved with purified water at room temperature (fluid extract:purified water volume ratio 1:4), centrifuged for 20min (speed 4000r / min), and the supernatant was prepared into a suitable concentration as the loading solution.

[0034] 2. Pretreatment of macroporous resin

[0035] Soak the pharmaceutical-grade resin HPD400, which was optimized by the static adsorption and elution experiment, in ethanol for 24 hours, then wash the resin with ...

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Abstract

The present invention relates to a column temperature controlling high borosilicate glass interlayer chromatography column, wherein the upper end of a chromatography column body is provided with a material inlet, a sand core is arranged inside the chromatography column body, the lower end of the chromatography column body is provided with a material outlet pipe, the material outlet pipe is provided with a material outlet control valve, the diameter of the material outlet pipe is less than the diameter of the chromatography column body, a jacket layer is arranged on the outer periphery of the chromatography column body, the outer periphery of the chromatography column body is completely surrounded by the jacket layer, a cavity is formed between the jacket layer and the chromatography column body, the jacket layer is provided with a carrier inlet and a carrier outlet, and the temperature of a heating agent is maintained at the required temperature T DEG C during the chromatography process so as to continuously supply heat for the filler in the chromatography column body and the elution solvent. According to the present invention, with the column temperature controlling high borosilicate glass interlayer chromatography column, the column separation column temperature can be well controlled, the non-room temperature separation of the temperature sensitive specific component can achieved, the separation efficiency of the soluble components in the hot solvent can be improved, and the column temperature controlling high borosilicate glass interlayer chromatography column are widely used in the column chromatography separation process requiring the column temperature control in the fields of chemistry, chemical industry and pharmacy, and has promotion values.

Description

technical field [0001] The invention relates to a high borosilicate glass interlayer chromatographic column for controlling the column temperature, which belongs to the extraction, separation and purification devices of organic compounds and natural products in the fields of chemistry, chemical industry and pharmacy. It is especially involved in the very temperature column separation of a class of special organic compounds and natural products that are sensitive to temperature. Background technique [0002] Chromatographic column is the main body in column chromatography separation technology, generally using glass tube or plexiglass tube. In the chemical, chemical and pharmaceutical fields, some substances are more sensitive to temperature, such as glycyrrhizic acid, a triterpenoid saponin component in natural products, is easily soluble in hot water, and column chromatography under heating conditions is beneficial to the separation of such components . A common glass chr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/22
Inventor 见玉娟刘明言
Owner ANHUI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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