Enterococcus faecium E9-10, screening and identification method thereof and application of enterococcus faecium E9-10 to degrading erythromycin and roxithromycin

A technology of Enterococcus faecium and roxithromycin, which is applied in the fields of microorganisms, screening and identification and application, can solve the problems of human intestinal bacterial flora imbalance, change the microecological environment of human intestinal flora, etc., and reduce secondary pollution. , low cost, cost reduction effect

Active Publication Date: 2015-09-30
潍坊中创生物科技有限公司
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  • Application Information

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Problems solved by technology

Long-term use will change the micro-ecological environment of the human intestinal flora, causing the

Method used

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  • Enterococcus faecium E9-10, screening and identification method thereof and application of enterococcus faecium E9-10 to degrading erythromycin and roxithromycin
  • Enterococcus faecium E9-10, screening and identification method thereof and application of enterococcus faecium E9-10 to degrading erythromycin and roxithromycin
  • Enterococcus faecium E9-10, screening and identification method thereof and application of enterococcus faecium E9-10 to degrading erythromycin and roxithromycin

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Embodiment

[0064] Example, a strain of Enterococcus faecium (Enterococcus faecium) E9-10 was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms, and its preservation number is: CGMCC No. 10575.

[0065] The above screening method for Enterococcus faecium (Enterococcus faecium) E9-10 comprises the following steps:

[0066] 1 Experimental materials

[0067] 1.1 Experimental strains

[0068] Sample source

[0069] Collect soil samples from different locations in Weifang, Yantai and other regions

[0070] 1.2 Experimental Instruments

[0071] Vertical pressure steam sterilizer (Hirayama HVE-50, Japan); biological safety cabinet (XUN BSC-1300ⅡA / B3); microscope (OLYMPUS CX21); high performance liquid chromatography (Aglient 1260); high performance liquid chromatography / mass spectrometry / Mass spectrometer (Aglient 1260-6430); analytical balance (Mettler Toledo PL 602-S, Switzerland); constant temperature incubator (MMM Incucell 111, German...

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Abstract

The invention discloses enterococcus faecium collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number being CGMCC No.10575. The enterococcus faecium has the technical effects that lactobacillus with an erythromycin and roxithromycin degrading function is screened from soil through preliminary screening and secondary screening of an antibiotic selective medium; a reasonable and effective screening method of an erythromycin and roxithromycin degrading strain is established to obtain lactobacillus meeting the needs; the safe and effective erythromycin and roxithromycin degrading strain is screened through separation, purification and screening of lactobacillus and a series of work, such as preliminary screening and secondary screening of erythromycin and roxithromycin degrading lactobacillus; when applied to control over erythromycin and roxithromycin pollution, the enterococcus faecium has the advantages of no secondary pollution, high treatment efficiency, wide range of application and low cost; the enterococcus faecium provides more choices for microbiological control over antibiotic pollution of complex environments.

Description

technical field [0001] The invention relates to a strain of microorganism, screening identification and application, in particular to a strain of Enterococcus faecium E9-10 and its screening identification and application in degrading erythromycin and roxithromycin, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Lactic acid bacteria are mainly distributed in a slightly acidic environment rich in mineral and organic nutrients. Dairy products, meat products, kimchi, fruit wine, vegetables and other materials are rich in lactic acid bacteria. In addition, animal digestive tracts are also important sources of lactic acid bacteria. Lactic acid bacteria play an important role in the nutrition and health of animals. They have the effects of antibacterial and anti-inflammatory, anti-oxidation, anti-tumor, lowering cholesterol, and improving immunity. [0003] Macrolide antibiotics have outstanding antibacterial activity...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12N1/02C12Q1/04
CPCA62D3/02A62D2101/28B09C1/10C12N1/20C12N1/205C12R2001/46
Inventor 郝莹孙军张金玲李凯马荣桧
Owner 潍坊中创生物科技有限公司
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