Novel method for separating/enriching trace roxithromycin in environment

Abstract

The invention provides a novel method for separating/enriching trace roxithromycin in environment. The method comprises the following steps: adding 0.4mg.mL<-1> researched roxithromycin test solution in a mouth polished colorimetric tube, wherein the volume of the roxithromycin test solution accounts for 1/50 of total volume of total solution in sublation; adding solid salt to adjust the ionic strength of the solution, shaking the solution to ensure that the solution can be sufficiently dissolved, and adjusting pH to be between 10.8 and 11.8, wherein the salt is solid salt of Na2CO3, (NH4)2SO4, NaH2PO4, MgSO4, Na2SO3 and NaOH; transferring the solution into a sublation column, adding the sublation solvent ionic liquid [Bmim]BF4 into the sublation column, and bringing to total volume by adding water, wherein the volume of the ionic liquid [Bmim]BF4 accounts for 1/25 of total volume of total solution in sublation, and the sublation flow rate of N2 is between 5 and 25mL.min<-1>; floating for 5 to 60 minutes, standing for 1 minute after aeration is stopped; taking out upper enriched [Bmim]BF4 phase by a burette when no air bubble exists; adding a certain amount of 75 percent sulphuric acid and 12 to 19 mL/mg of ROX into the sublation column to perform a color development reaction at room temperature for 0 to 70 minutes; analyzing and determining the content of the solution by using a molecule fluorescence photometry in 350nm excitation wavelength and 508nm emission wavelength. The method optimizes the best condition of sublation, and carries out separation/enrichment analysis on the trace roxithromycin in an actual environmental water sample by combining the molecule fluorescence photometry under an optimized testing condition.

Description

technical field [0001] The invention relates to a method for separating / enriching trace amounts of roxithromycin (ROX) in the environment, specifically referring to 1-butyl-3-methylimidazolium tetrafluoroborate ionic liquid ([Bmim]BF 4 ) is the flotation agent, with sodium carbonate as the salting-out agent of phase separation, in the inert gas N 2 A method for separating / enriching trace amounts of roxithromycin in the environment based on ionic liquid aqueous two-phase solvent flotation (Ionic liquid aqueous two-phase solventsublation, ILATSS). Background technique [0002] The environmental exposure of pharmaceuticals and personal care products (PPCPs) pollutants has been increasingly concerned by people. Among them, antibiotics are widely used in aquaculture, agriculture and clinical practice, which makes the residual antibiotics in the environment Topics in analysis, migration, bacterial resistance, and impacts on humans and biomes have become a hot topic for environmen...

AI Technical Summary

Problems solved by technology

[0006] The present invention overcomes the shortcomings of benzene, toluene, xylene, isoamyl alcohol, etc. commonly used in air flotation solvent flotation in the prior art, which will cause secondary pollution to the environment, and affect the accuracy due to the volatility of the solvent. The extraction efficiency of ionic liquid two-phase aqueous phase (enrichment multiple is related to phase ratio) is limited, the enrichment multiple can only reach 1 to 2 orders of magnitude, and the enrichment of ultra-trace samples requires multiple extractions. A new method is proposed. Separation / enrichment method (Ionic liquid aqueous two-phase solvent sublation, ILATSS), which integrates ionic liquid aqueous two-phase extraction and air flotation solvent flotation, has a high enrichment factor and high selectivity, and the components in the separation process It has nothing to do with the phase, non-toxic, non-polluting, good separation effect, good selectivity, can process a large number of low-concentration components at the same time, and has the characteristics of simple equipment and easy operation.

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