Pediococcus acidilactici P3-4, screening and identification method thereof and application of pediococcus acidilactici P3-4 to degrading spiramycin

A technology of Pediococcus lactis and spiramycin, applied in application, bacteria, microorganism-based methods, etc., can solve the problems of human intestinal bacterial flora imbalance, change the micro-ecological environment of human intestinal flora, etc. Sub-pollution, low cost, cost reduction effect

Active Publication Date: 2015-09-30
山东中创健康科技集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long-term use will change the micro-ecological environment of the human intestinal flora, causing the imbalance of the human intestinal bacterial flora, and at the same time prone to bacterial resistance

Method used

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  • Pediococcus acidilactici P3-4, screening and identification method thereof and application of pediococcus acidilactici P3-4 to degrading spiramycin
  • Pediococcus acidilactici P3-4, screening and identification method thereof and application of pediococcus acidilactici P3-4 to degrading spiramycin
  • Pediococcus acidilactici P3-4, screening and identification method thereof and application of pediococcus acidilactici P3-4 to degrading spiramycin

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Effect test

Embodiment

[0054] Example, a strain of Pediococcus lactis ( Pediococcus acidilactici ) P3-4, preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and its preservation number is: CGMCC No.10574.

[0055] Pediococcus lactis above ( Pediococcus acidilactici ) The screening method of P3-4, comprising the following steps:

[0056] 1 Experimental materials

[0057] 1.1 Experimental strains

[0058] Sample source

[0059] Collect soil samples from different locations in Weifang, Yantai and other regions

[0060] 1.2 Experimental Instruments

[0061] Vertical pressure steam sterilizer (Hirayama HVE-50, Japan); biological safety cabinet (XUN BSC-1300ⅡA / B3); microscope (OLYMPUS CX21); high performance liquid chromatography (Aglient 1260); high performance liquid chromatography / mass spectrometry / Mass spectrometer (Aglient 1260-6430); analytical balance (Mettler Toledo PL 602-S, Switzerland); constant temperature incubator (MMM Incucell 111, Ge...

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Abstract

The invention discloses pediococcus acidilactici collected in the China General Microbiological Culture Collection Center (CGMCC), with the collection number of CGMCC No.10574. The pediococcus acidilactici has the technical effects that a new method for screening lactobacillus is established by adopting the mode of combining a microbiological method with a chemical method; lactobacillus with a spiramycin degrading function is screened from soil through preliminary screening and secondary screening of an antibiotic selective medium; a reasonable and effective screening method of a spiramycin degrading strain is established to obtain lactobacillus meeting the needs; the safe and effective spiramycin degrading strain is screened through separation, purification and screening of lactobacillus and preliminary screening and secondary screening of spiramycin degrading lactobacillus; when applied to control over spiramycin pollution, the pediococcus acidilactici has the advantages of no secondary pollution, high treatment efficiency, wide range of application and low cost; the pediococcus acidilactici provides more choices for microbiological control over antibiotic pollution of complex environments.

Description

technical field [0001] The invention relates to a strain of microorganism, screening identification and application, in particular to a strain of Pediococcus lactis P3-4 and its screening identification and application in degrading spiramycin, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Lactic acid bacteria are mainly distributed in a slightly acidic environment rich in mineral and organic nutrients. Dairy products, meat products, kimchi, fruit wine, vegetables and other materials are rich in lactic acid bacteria. In addition, animal digestive tracts are also important sources of lactic acid bacteria. Lactic acid bacteria play an important role in the nutrition and health of animals. They have the effects of antibacterial and anti-inflammatory, anti-oxidation, anti-tumor, lowering cholesterol, and improving immunity. [0003] Macrolide antibiotics have outstanding antibacterial activity against Gram-positive ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/015B09C1/10A62D3/02C12R1/01A62D101/28
Inventor 郝莹孙军田国宁宫小明王洪涛
Owner 山东中创健康科技集团有限公司
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