Alpha-fetoprotein variant AFP-L3 quantitative detection kit and use thereof
An AFP-L3, quantitative detection technology, used in biological testing, measuring devices, material inspection products, etc., to achieve the effect of simple application, strong stability, and improved stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] The preparation of embodiment 1 kit
[0033] The kit of the present invention consists of: AFP-L3 negative and 1 bottle of positive control; AFP monoclonal antibody coated plate (96 holes); 1 bottle of AFP monoclonal antibody solution labeled with horseradish peroxidase (HRP), 6ml / 1 bottle of lentil agglutinin (LCA) solution; 1 bottle of control buffer; 1 bottle of AFP standard, 1 bottle of substrate solution and 1 bottle of chromogenic solution, each 5ml / bottle; 1 bottle of reaction termination solution, 5ml / bottle; Wash buffer (20X concentrated) 1 bottle, 30ml / bottle.
[0034] The enzyme label plate adopts imported or domestically produced 12×8 detachable strips. Dilute the AFP monoclonal antibody to 20 μg / ml with 0.05mol / L carbonate buffer, add to each well of the enzyme labeling plate, 100 μl per well, absorb overnight, wash the plate with washing buffer, and then block with the blocking buffer Spin it overnight, dry it in the air, and obtain the monoclonal antib...
Embodiment 2
[0044] Example 2 Sensitivity investigation of the kit
[0045] Prepare PBS buffer solution with different concentrations of AFP-L3 standard substance respectively, the concentrations are respectively 1ng / ml, 5ng / ml, 10ng / ml, 15ng / ml, 20ng / ml, and the kit prepared in Example 1 is used for detection, to control The buffer solution is used as a blank control, and the specific detection method is as follows:
[0046] Washing buffer preparation: dilute the concentrated washing buffer provided in the kit with distilled water 20 times.
[0047] a) Antigen-antibody reaction: Add 50 μl of AFP-L3 standard solution and control buffer to the microwells of the antibody-coated plate provided in the kit, respectively, and use a marker pen to mark them into series 1 and 2. Incubate in a water bath at 37°C for 50 minutes. Wash the plate 5 times.
[0048] b) A solution containing lectin is added to the 1st series of sample wells, and a control buffer solution not containing lectin is added t...
Embodiment 3
[0058] Example 3 Stability investigation of the kit
[0059] After the kit prepared in Example 1 was placed at 20° C. for 6 months and 12 months, respectively, the sensitivity of the kit was measured according to the method in Example 2.
[0060] In this embodiment, the comparison ratio is set as follows:
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More 
PatSnap Eureka turns technology decisions into work you can execute. Powered by our Innovation Knowledge Graph, it runs expert workflows across engineering, life sciences, materials and intellectual property. Get your review-ready output in minutes.
