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Application of uraria for promoting osteogenesis or providing neuro-protection

A technology for neuroprotection and brisket, applied in the field of promoting bone formation or providing neuroprotection, can solve problems such as no reports showing bristle and skeletal diseases.

Active Publication Date: 2015-10-14
TAIPEI MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, there are no reports linking rabbittail to bone disease

Method used

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  • Application of uraria for promoting osteogenesis or providing neuro-protection
  • Application of uraria for promoting osteogenesis or providing neuro-protection
  • Application of uraria for promoting osteogenesis or providing neuro-protection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0084] Materials and Methods

[0085] plant material

[0086] The roots of Uraria crinite were collected from Mingjian Township Farmers Association, Nantou County, Taiwan, and identified by Mr. Guo Zhaolin, Department of Chinese Pharmacy and Chinese Medicine Resources, China Medical University, Taichung, Taiwan. One specimen (M-343) was deposited at the Institute of Pharmacy, Taipei Medical University, Taiwan.

[0087] Reagents and Chemicals

[0088] DMSO (dimethyl sulfoxide), thiazolium blue tetrazolium bromide (MTT), Triton X-100 (Triton X-100), disodium p-nitrophenyl phosphate matrix, Alizarin Red-S (Alizarin Red -S), cetylpyridinium chloride monohydrate (cetylpyridinium chloride monohydrate), ascorbic acid, and β-glycerophosphate disodium salt hydrate were purchased from Sigma-Aldrich Company (Sigma, St. Louis, Missouri, USA). Phosphate buffered saline (PBS) and trypsin were purchased from Gibco (Gibco Canada Inc., Burlington, Ont., Canada). The rotational brightne...

example 1

[0106] Example 1 Rabbit's tail grass promotes osteoblast differentiation and matrix mineralization in Hob cells

[0107] The 95% ethanol (EtOH) extract from the root of Rabbittail Grass (UC) was extracted and chromatographed using biological grade methods. 95% ethanol crude extract was suspended in H 2 O and divided sequentially with n-hexane, ethyl acetate and n-butanol to obtain 4 fractions. The cell proliferation of the crude extract in Hob cells was evaluated by MTT method. The results showed that none of the crude extracts were found to cause significant cytotoxicity or proliferation of HOb cells at concentrations of 100 μg / ml or below (data not shown). ALP activity is the main marker of osteoblast differentiation. The effect of the extract on osteoblast differentiation was detected by measuring the ALP activity of HOb cells. After Hob cells were treated for 72 hours, the results showed that the 95% ethanol crude extract and the aqueous fraction of 100 μg / ml showed ...

example 2

[0108] Example 2 Isolation and Identification of Active Compounds from Rabbittail Grass Bioguidance

[0109] The active ethyl acetate and n-butanol fractions were introduced into various column chromatography and purified by HPLC and yielded a new compound (3), as well as 10 known compounds, 2', 4', 5, 7 -tetrahydroxyisoflavanone (dalbergioidin) (1) (Durango, Quinones, Torres, Rosero, Gil & Echeverri, 2002), salicylic acid (2) (Scott, 1972), vitexin (vitexin) (4) (Krafczyk & Glomb , 2008), tryptophan (tryptophan) (5) ((Lee&Phillips, 1992)), maltol-3-O-β-D-glucopyranoside (maltol-3-O-β-D-glucopyranoside) ( 6) (Ono, Masuoka, Tanaka, Ito & Nohara, 2001), adenosine (7) (Moyroud & Strazewski, 1999), spatholosineside A (8) (Yin, Liu, Wang, Tu, Liang & Zhao, 2008), byzantionoside B (9) (Matsunami, Otsuka & Takeda, 2010), (7R, 8R)-threo-guaiacylglycerol-8-O-4'-sinapyl ether7-O-β-D-glucopyranoside ((7R, 8R)-threo- guaiacylglycerol-8-O-4′-sinapyl ether7-O-β-D-glucopyranoside) (10) (Ma...

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PUM

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Abstract

The invention provides a method of promoting osteogenesis, increasing bone quality or increasing strong bone growth rate. The method includes a step of feeding uraria, or a part of the uraria, or an extract of the uraria to an individual. The invention also provides a method of treating and / or preventing osteoporosis and a method of providing neuro-protection, wherein the methods includes feeding the uraria, or a part of the uraria, or the extract of the uraria to the individual.

Description

technical field [0001] The present invention relates to a method of using plant products to promote osteogenesis or provide neuroprotection. In particular, the present invention uses Uraria to promote osteogenesis or provide neuroprotection. Background technique [0002] Bone is the structural material of the body skeleton and serves to maintain essential bone mass and structure. Bone contains calcium ions (Ca2+) and plays an important role in maintaining the amount of calcium in the blood. For this reason, bone growth is a metabolic balance between the activities of osteoblasts and osteoclasts in the bone remodeling cycle. When the balance between bone resorption and bone formation is disturbed, the amount of bone tissue replaced by osteoblasts does not match the amount of bone tissue resorbed by osteoclasts, resulting in osteoporosis, a common disease that causes loss of bone density or mass. disease. Osteoblasts are bone-forming cells derived from bone marrow mesenchy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48C07H17/04A61P19/08A61P25/00A61P19/10A61P19/02A61P9/10A61P25/02A61P25/16A61P25/28A61K125/00
Inventor 李美贤
Owner TAIPEI MEDICAL UNIV
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