Early diagnosis method of ventilator-induced lung injury

A technology of mechanical ventilation and early diagnosis, applied in biochemical equipment and methods, microbiological measurement/inspection, material inspection products, etc., can solve problems such as difficult diagnosis

Inactive Publication Date: 2015-10-14
郭永清
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  • Abstract
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Problems solved by technology

[0003] The invention provides a method for early diagnosis of mechanical ventilation lung injury, whi

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  • Early diagnosis method of ventilator-induced lung injury

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Embodiment Construction

[0011] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0012] Embodiments of the present invention provide a method for early diagnosis of mechanically ventilated lung injury, such as figure 1 shown, including:

[0013] Step 101, obtaining lung epithelial cell tissue, and placing the lung epithelial cell tissue in RPMI-1640 medium for culturing;

[0014] Wherein, the lung epithelial cell tissue was cultured in RPMI1640, and the culture medium was cultured and loaded in a CO2 incubator, wherein the temperature of the incubator was 37° C., and the CO2 content was 5%.

[0015] Step 102, measuring the expression of plasminogen activator inhibitor-1PAI-1;

[0016] Among them, plasminogen activator inhibitor-1 (plasminogen activator inhibitor-1, PAI-1) is the main inhibitor of urokinase t...

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Abstract

The invention relates to the field of early diagnosis of ventilator-induced lung injury, and discloses an early diagnosis method of ventilator-induced lung injury, which comprises the following steps: acquiring a lung epithelial cell tissue, culturing the lung epithelial cell tissue in an RPMI-1640 culture medium, determining the expression condition of the profibrinolysin activator inhibitor-1 (PAI-1), and carrying out diagnosis on ventilator-induced lung injury according to the expression condition of the PAI-1. The method can perform early diagnosis on ventilator-induced lung injury by using the PAI-1 as the biological index for monitoring ventilator-induced lung injury.

Description

technical field [0001] The invention relates to the field of early diagnosis of mechanical ventilation lung injury, in particular to an early diagnosis method of mechanical ventilation lung injury. Background technique [0002] In the treatment of respiratory failure caused by acute lung injury (ALI) and acute respiratory distress syndrome (ARDS), mechanical ventilation (MV) is an essential means One, and mechanical ventilation is often used during general anesthesia for surgery. However, mechanical ventilation will bring negative effects. It will aggravate the damage of the original lung and even induce damage to the healthy lung. This phenomenon is called mechanical ventilation lung injury (ventilator-induced lung injury, VILI). Generally speaking, mechanical injury occurs in the early stage of VILI, followed by biological injury mediated by inflammatory cells and cytokines. Mechanical injury can cause biological injury, and biological injury can also aggravate mechanica...

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Application Information

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IPC IPC(8): C12Q1/68G01N33/58G01N15/14
CPCC12Q1/6883C12Q2600/158G01N15/14G01N33/582
Inventor 郭永清张玮玮卫建峰
Owner 郭永清
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