Early diagnosis method of ventilator-induced lung injury
A technology of mechanical ventilation and early diagnosis, applied in biochemical equipment and methods, microbiological measurement/inspection, material inspection products, etc., can solve problems such as difficult diagnosis
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[0011] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0012] Embodiments of the present invention provide a method for early diagnosis of mechanically ventilated lung injury, such as figure 1 shown, including:
[0013] Step 101, obtaining lung epithelial cell tissue, and placing the lung epithelial cell tissue in RPMI-1640 medium for culturing;
[0014] Wherein, the lung epithelial cell tissue was cultured in RPMI1640, and the culture medium was cultured and loaded in a CO2 incubator, wherein the temperature of the incubator was 37° C., and the CO2 content was 5%.
[0015] Step 102, measuring the expression of plasminogen activator inhibitor-1PAI-1;
[0016] Among them, plasminogen activator inhibitor-1 (plasminogen activator inhibitor-1, PAI-1) is the main inhibitor of urokinase t...
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