Method for biosynthesizing sterol ester through esterification and ester interchange in vacuum solvent-free system

A biosynthesis, solvent-free technology, applied in the direction of fermentation, to achieve the effect of avoiding harmful solvent residues, simple process and low production cost

Inactive Publication Date: 2015-10-28
SOYBEAN TECH DEV RES CENT HEILONGJIANG PROV
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Problems solved by technology

Katsunori et al., Qiu Aiyong, etc. respectively used lipase to catalyze the reaction of sterols and unsaturated fatty acids in aqueous solvent systems to pr...

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  • Method for biosynthesizing sterol ester through esterification and ester interchange in vacuum solvent-free system
  • Method for biosynthesizing sterol ester through esterification and ester interchange in vacuum solvent-free system
  • Method for biosynthesizing sterol ester through esterification and ester interchange in vacuum solvent-free system

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Embodiment Construction

[0018] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.

[0019] The invention provides a method for esterification and transesterification biosynthesis of sterol esters in a vacuum solvent-free system. The method is studied under vacuum, solvent-free, and water-free reaction conditions, using immobilized lipases Lipozyme IM and Novozym 435 is used as a catalyst to biosynthesize phytosterol esters, explore the method of esterification and transesterification of oleic acid, fatty acid methyl esters, and oils with phytosterols to synthesize phytosterol esters, and investigate the reaction under the conditions of ...

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Abstract

A method for biosynthesizing sterol ester through esterification and ester interchange in a vacuum solvent-free system is characterized in that corresponding long-chain acyl ester is generated through esterification of sterol and oleic acid and ester interchange of methyl oleate and triglyceride at a high conversion rate under vacuum solvent-free added water-free reaction conditions with immobilized lipase Lipozyme IM and Novozym 435 as a catalyst. Ester interchange of sterol and methyl oleate is carried out under optimum reaction conditions, and the immobilized lipase Lipozyme IM still has a conversion rate being greater than 90% even after the immobilized lipase Lipozyme IM is repeatedly used 10 times, and the lipase Novozym 435 still has a conversion rate being greater than 40% after the lipase Novozym 435 is repeated used 10 times.

Description

technical field [0001] The invention relates to a method for synthesizing phytosterol esters. Background technique [0002] Phytosterol is a steroidal compound, which has important physiological functions, especially in lowering cholesterol, anti-cancer, anti-virus, immune regulation, etc. It has been hailed as "the key to life" by the scientific community. Many studies have proved that supplementing phytosterols has the effect of inhibiting cholesterol absorption, and can significantly reduce the content of total cholesterol and low-density lipoprotein cholesterol (bad cholesterol, LDL) in the blood without reducing high-density lipoprotein cholesterol (good cholesterol, HDL) and triglyceride content. The research report of the U.S. Food and Drug Administration (FDA) shows that a daily intake of 1.3g of phytosterol esters can reduce cholesterol. Phytosterols are one of the top ten functional nutritional ingredients recommended by the International Society of Nutrition in ...

Claims

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Application Information

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IPC IPC(8): C12P33/02
Inventor 陈昊许慧胡立志于殿宇孙树坤朱秀清任悦张旭齐晓芬刘欣张青
Owner SOYBEAN TECH DEV RES CENT HEILONGJIANG PROV
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