36 results about "Novozym 435" patented technology

The invention relates to a method for preparing R-2-naphthylethylamine by dynamic kinetic resolution. Using 2‑naphthylethylamine as raw material, Novozym 435 as resolution catalyst, D‑(‑)‑O‑acetylmandelic acid as acyl donor, KT‑02 (nickel catalyst) as racemization catalyst, in autoclave 2-Naphthylethylamine is completely converted to obtain (R)-(1-(2-naphthyl)ethyl)acetamide (ee value 99%); acidolysis is carried out after amide purification to obtain R-2 ‑Naphthylethylamine salt, the salt is then basified, extracted, dried, concentrated, etc. to obtain R‑2‑naphthylethylamine. The product yield of each step can reach more than 90%, and the ee value is greater than 99%. The invention has the characteristics of cheap and easy to obtain racemic catalyst, complete utilization of raw materials, good product yield, high optical purity and the like. In the production and preparation of R-2-naphthylethylamine, it has great guidance and application value.

The invention relates to a method for preparing R-2-naphthylethylamine by dynamic kinetic resolution. Using 2-naphthylethylamine as raw material, Novozym 435 as a resolution catalyst, R-1-phenylethyl alcohol acetate as an acyl donor, and Raney nickel as a racemization catalyst, hydrogen is introduced into the autoclave to react, 2- Complete conversion of naphthylethylamine to obtain (R)-(1-(2-naphthyl)ethyl)acetamide (ee value 99%); acid hydrolysis after purification of the amide to obtain R-2-naphthylethylamine salt, which is passed through Operations such as alkalization, extraction, drying, and concentration can obtain R-2-naphthylethylamine, and the product yield and ee value of the whole steps can reach more than 90%. The invention has the characteristics of cheap and easy to obtain racemic catalyst, complete utilization of raw materials, good product yield, high optical purity and the like. In the production and preparation of R-2-naphthylethylamine, it has great guidance and application value.

The invention discloses a method for preparing a main intermediate of dabigatran etexilate through enzymatic reaction. The main steps are as follows: first, 3-[4-methylamino-3-amino-N-(2-pyridyl)-benzamido]-ethyl acrylate and 2-(4-cyano aniline) acetic acid are added in a reaction solvent, mixed and dissolved; immobilized enzyme Novozym 435 is added and reaction is carried out; second, after the reaction is finished, the immobilized enzyme Novozym 435 is removed through filtration, an organic phase layer and a filtrate are obtained; third, the obtained organic layer is subjected to solvent evaporation, and an oil-like substances are obtained, acetic acid is added and a reflux reaction is carried out; fourth, after the reaction is finished, acetic acid is evaporated, ethyl acetate and an alkaline solution are added, washing and extraction are carried out, the obtained organic layer is subjected to drying by distillation, and 3-[[[2-[[(4-cyano phenyl)amino] methyl]-1-methyl-1H-benzimidazole-5-group] carbonyl] pyridine-2-group amino] ethyl propionate ethyl ester. The method has simple operations and mild reaction conditions, the product with high purity is easy to obtain, the yield is high and the method is suitable for industrial amplification production.

The invention belongs to the biotechnology field of non-aqueous phase enzymatic organic synthesis application, and particularly relates to an ester enzyme method production process for an azeotropic dewatering coupling simulated moving bed. The process is applied to the synthesis of various ester bonds by catalysis of a biological catalyst (such as Novozym 435). The invention provides a commonly used ester enzyme method synthesis novel process; in the premise of not affecting the catalytic activity of the biological catalyst, the process can continuously remove water by decompression azeotropy and make the reaction balance move towards the ester synthesis, thereby greatly improving the conversion rate of the enzymatic organic synthesis, ensuring the complete reaction and thoroughly solving the continuous dewatering problem in the non-aqueous phase enzymatic organic synthesis; by the coupling of the enzymatic reaction system and the simulated moving bed chromatographic separation system, the process can ensure the complete separation of reaction products and excessive reaction materials, solve the problem of recycling the excessive reaction materials, improve the conversion rate ofraw materials, the product yield and the product purity to be nearly 100 percent, greatly lower production cost, realize the zero emission of waste water and waste residue during the production of ester chemical products and has wide application prospect in fields such as medicine, food, material, fine chemical industry.

The invention discloses a preparation method of (S)-tert-butyldimethylsiloxy-glutaric acid monobenzyl monoamide, which belongs to the field of medicine. The method uses the compound (S)-4-cyano-3-hydroxybutyrate as a raw material, catalyzed by Novozymes lipase 435, and benzyl alcohol is transesterified to obtain (S)-4-cyano-3-hydroxybutyrate Benzyl ester, combined with TBSCl again, obtains TBS protection (S)-4-cyano-3-tert-butyldimethylsiloxane monobenzyl butyrate, and then oxidizes to obtain S)-3-tert-butyldimethyl Siloxyglutarate monobenzyl monoamide. Using NOVO‑435 to carry out transesterification to obtain benzyl ester has a higher yield than the original process, less waste, and avoids the pollution of titanium tetrachloride. Use hydrogen peroxide and alkali or catalytic oxidation of amides, less waste, and avoid the high-pollution oxidation method of manganese dioxide, and avoid the high-cost method of catalytic oxidation with Wilkinson precious metals. The process cost is lower and industrialization is easier.

The invention relates to a method for preparing R-1-phenethylamine through dynamic kinetic resolution. Using 1‑phenethylamine as raw material, Novozym 435 as resolution catalyst, D‑(‑)‑O‑acetylmandelic acid as acyl donor, KT‑02 (nickel catalyst) as racemization catalyst, in autoclave 1-Phenylethylamine is completely converted to obtain (R)-(1-Phenylethyl)acetamide (ee value 99%); acidolysis is carried out after purification of the amide to obtain R-1-Phenylethylamine Salt, the salt is then subjected to operations such as alkalization, extraction, drying, and concentration to obtain R-1-phenylethylamine, and the product yield and ee value of the whole step can reach more than 90%. The invention has the characteristics of cheap and easy-to-obtain racemization catalyst, complete utilization of raw materials, good product yield, high purity and the like. In the production and preparation of R-1-phenethylamine, it has great guidance and application value.

The invention relates to synthesis of medicinal biodegradable poly(epsilon-caprolactone) and an application method thereof in the field of high polymer chemistry technique. The medicinal biodegradable poly (epsilon-caprolactone) can be obtained by separating and purifying the reactant after epsilon-caprolactone and immobilized lipase Novozym 435 are mixed and reacted at the temperature of between60 and 80 DEG C for 2 to 48 hours by using a mass polymerization method or a solution polymerization method in a closed reaction system. Due to the adoption of the immobilized lipase Novozym 435 which serves as a catalyst, the biodegradable poly (epsilon-caprolactone) can be prepared by the method which has the advantages of no catalyst residue and easy control of conditions.