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Enzymatical method for synthesizing water-soluble ferulic acyl glyceride

A technology of feruloyl glyceride and feruloyl glycerol, which is applied in the field of enzymatic synthesis of water-soluble feruloyl glyceride, can solve the problems of long reaction time and low product yield, and achieve the effect of increasing yield

Inactive Publication Date: 2008-06-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is only one study on the synthesis of water-soluble feruloylglycerides, but the study is carried out in a solvent environment, and the reaction time is long, and the yield of the product is low

Method used

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  • Enzymatical method for synthesizing water-soluble ferulic acyl glyceride

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Experimental program
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Effect test

Embodiment 1

[0035]The raw material quality indicators used are: ferulic acid (purity>99%), ethyl ferulate (purity>99%), glycerin (purity>99%), Novozym 435 (Denmark), Lipozyme RM IM (Denmark), LipozymeTL IM (Denmark), porcine pancreatic lipase (sigma).

[0036] (1) Screening of lipase, screening was performed from Novozym 435 (Denmark), Lipozyme RM IM (Denmark), Lipozyme TL IM (Denmark), and porcine pancreatic lipase (sigma). The results showed that Novozym435 (Candida antarctica lipase B) was the only lipase active on ethyl ferulic acid and feruloyl glyceride substrates. Therefore, we chose Novozym 435 as the lipase for the synthesis of feruloyl oleoyl glyceride.

[0037] (2) The establishment of the synthetic route, the esterification reaction of ferulic acid and glycerin, the transesterification reaction of ethyl ferulic acid and glycerol, the reactor is a water bath shaker, and all the other conditions are the same as above. As a result, the transesterification reaction route of ethy...

Embodiment 2

[0050] Feruloylglycerides were synthesized by transesterification of ethyl ferulate and glycerol in a vacuum rotary bioreactor under the action of Novozym 435 lipase;

[0051] The amount of enzyme added is 1% (w / w) based on the total substrate (total weight of ethyl ferulate and glycerol),

[0052] Substrate ratio: ethyl ferulate / glycerol (w / w) is 0.05:1,

[0053] Speed ​​from 150rpm,

[0054] The reaction temperature was 80°C, and the reaction time was 10 hours.

[0055] The conversion rate of ethyl ferulic acid reaches 100% and the yield of feruloyl glyceride reaches more than 90%.

Embodiment 3

[0057] Feruloylglycerides were synthesized by transesterification of ethyl ferulate and glycerol in a vacuum rotary bioreactor under the action of Novozym 435 lipase;

[0058] The amount of enzyme added is 16% (w / w) based on total substrate (total weight of ethyl ferulate and glycerol),

[0059] Substrate ratio: ethyl ferulate / glycerol (w / w) is 0.5:1,

[0060] Speed ​​from 50rpm,

[0061] The reaction temperature was 50°C, and the reaction time was 10 hours.

[0062] The conversion rate of ethyl ferulic acid reaches 100% and the yield of feruloyl glyceride reaches more than 90%.

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Abstract

An enzymic synthesis method of an oil-soluble feruloyl glyceride belongs to the technical field of the comprehensive utilization of the agricultural byproduct ferulic acid. The present invention synthesizes the oil-soluble feruloyl glyceride which has the functions of antioxidation and ultraviolet absorption by the reaction of ferulic acid ethyl ester and glycerin under the effect of Novozym 435 lipase by a pressure-reduction rotary biological reaction vessel. The present invention realizes that the transformation rate of the ferulic acid ethyl ester is 100 percent, and the yield of the feruloyl glyceride is 96 percent; the reaction time is shortened by more than 60 hours than an ordinary method, which is shorted to be less than 10 hours from 70 to 144 hours; the yield is improved by 1.5 times; the present invention successfully solves the problem that the enzymic catalysis synthesizes the oil-soluble feruloyl glyceride hardly. The pressure-reduction rotary biological reaction vessel can eliminate the influence of an external mass transfer on the reaction velocity; by-product ethanol can be removed well under the pressure reduction; the stir during the reaction is from the self rotation of the reaction vessel, which avoids the damage effect to immobilized enzyme caused by directly contacting and prolongs the service life of the enzyme.

Description

technical field [0001] The invention discloses an enzymatic synthesis method of water-soluble feruloylglyceride, which belongs to the field of comprehensive utilization of ferulic acid of agricultural by-products such as rice bran and bran. Background technique [0002] The scientific name of ferulic acid is 4-hydroxy-3-methoxycinnamic acid, also known as 4-hydroxy-3-methoxy-2-phenylacrylic acid, and its content is extremely high in the seeds, leaves, and Chinese herbal medicine angelica of various plants. , others like rice, wheat, oats, coffee, pineapple are natural sources of ferulic acid. Ferulic acid has good antioxidant activity, and has a strong scavenging effect on hydrogen peroxide, superoxide radicals, hydroxyl radicals, and peroxynitroso. At present, Japan has allowed it to be used as a food additive. It can also be used as an antioxidant and function-promoting substance in sports foods, and it can also be gradually used as a cross-linking agent in the preparatio...

Claims

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Application Information

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IPC IPC(8): C12P7/62
Inventor 金青哲孙尚德单良刘元法王兴国
Owner JIANGNAN UNIV
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