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Method for synthesizing low-calorie structure lipid by using microwave treatment with assistance of enzymolysis

A low-calorie structured lipid and pretreatment technology, which is applied in fermentation and other directions, can solve the problem of low synthesis efficiency of low-calorie structured lipid, and achieve the effects of promoting industrial production, increasing frequency conversion movement, and increasing octanoic acid insertion rate

Inactive Publication Date: 2019-09-10
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for microwave pretreatment-assisted enzymatic synthesis of low-calorie structured lipids, which solves the problem of low synthesis efficiency of low-calorie structured lipids prepared by traditional enzymatic acid hydrolysis

Method used

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  • Method for synthesizing low-calorie structure lipid by using microwave treatment with assistance of enzymolysis
  • Method for synthesizing low-calorie structure lipid by using microwave treatment with assistance of enzymolysis
  • Method for synthesizing low-calorie structure lipid by using microwave treatment with assistance of enzymolysis

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Embodiment 1

[0018] A method for microwave pretreatment-assisted enzymatic synthesis of low-calorie structural lipids, comprising the following steps: (1) rapeseed oil and octanoic acid are in a molar ratio of 1:2, 1:4, 1:6, 1:8, 1:10 Mix, add 8% enzyme amount of immobilized Novozym 435 lipase, add 5mL n-hexane. (2) The microwave power is 200W, and the microwave pretreatment time is 60S. (3) The amount of enzyme added is 8%, the reaction temperature is 50° C., and the reaction time is 8 hours. (4) Add 0.5 mol potassium hydroxide. (5) Rotary evaporation temperature is 40°C. The effect of molar ratio on octanoic acid insertion rate was examined.

Embodiment 2

[0020] A method for microwave pretreatment-assisted enzymatic synthesis of low-calorie structural lipids, comprising the following steps: (1) rapeseed oil and octanoic acid are mixed in a 1:6 molar ratio, and the enzyme amount of immobilized Novozym 435 lipase is 8%, Add 5 mL of n-hexane. (2) Under different microwave power (100W, 200W, 300W, 400W, 500W), the microwave pretreatment time is 60S. (3) The amount of enzyme added is 8%, the reaction temperature is 50° C., and the reaction time is 8 hours. (4) Add 0.5 mol potassium hydroxide. (5) Rotary evaporation temperature is 40°C. The effect of microwave power on the insertion rate of octanoic acid was examined.

Embodiment 3

[0022] A method for microwave pretreatment-assisted enzymatic synthesis of low-calorie structural lipids, comprising the following steps: (1) rapeseed oil and octanoic acid are mixed in a 1:6 molar ratio, and the enzyme amount of immobilized Novozym 435 lipase is 8%, Add 5 mL of n-hexane. (2) Under the microwave power of 200W, microwave pretreatment time (30S, 60S, 90S, 120S, 150S). (3) The amount of enzyme added is 8%, the reaction temperature is 50° C., and the reaction time is 8 hours. (4) Add 0.5 mol potassium hydroxide. (5) Rotary evaporation temperature is 40°C. The effect of microwave pretreatment time on octanoic acid intercalation rate was examined.

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Abstract

The invention discloses a method for synthesizing a low-calorie structure lipid by using microwave treatment with the assistance of enzymolysis, and belongs to the field of preparation of structure lipids. The preparation method comprises the specific steps that 1, caprylic acid and rapeseed oil are weighed in a certain molar ratio and put into 50 mL conical flasks with covers, and a certain amount (based on the total substrate mass) of immobilized Novozym 435 lipase is added; 2, the conical flasks are placed in a microwave instrument and pretreated for a certain time at different microwave powers; 3, the conical flasks are placed in a constant temperature water bath oscillator at 200 r / min to carry out a reaction for different time at different reaction temperatures; 4, rotary evaporationis conducted at the temperature of 40 DEG C to remove an organic solvent, and a product is obtained. Through single factor experiments, the optimal material proportion, enzyme adding amount, microwave pretreatment time, microwave power, reaction temperature and reaction time are determined. The method solves the problem that a traditional method for synthesizing the low-calorie structure lipid through transesterification is low in synthesizing efficiency. In the method, microwave treatment is combined with enzymolysis, the method is simple in process and saves energy sources, and the reactionefficiency can be well improved.

Description

[0001] The invention relates to a microwave pretreatment-assisted enzymatic method for synthesizing low-calorie structural lipids, which belongs to the field of structural lipid preparation and is purified by alkali neutralization and rotary evaporation extraction. Background technique [0002] Edible oil is a triglyceride that can provide calories and fatty acids to the human body, and at the same time impart color, aroma and taste to food. It is generally divided into vegetable oil and animal oil. Vegetable oil is rich in unsaturated fatty acids and essential fatty acids, which play an important role in suppressing blood lipids and preventing chronic diseases. However, unsaturated fatty acids in vegetable oil will produce peroxidized substances, which in turn generate free radicals , aging and cancer; animal oil contains cholesterol indispensable for human metabolism, however, its essential fatty acid content is less. Therefore, it is a good way to use vegetable oil and anim...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCC12P7/6445
Inventor 李杨王中江郑丽和铭钰解长远
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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