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Method for preparing diglyceride from crude fish oil of marine aquatic fishes

A technology of marine aquatic products and diglycerides, which is applied in the direction of fermentation to achieve the effect of increasing utilization value, creating economic and social benefits, and promoting synthesis research

Active Publication Date: 2015-12-09
HUAQIAO UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, diacylglycerides prepared from fish oil of marine aquatic fish are rarely reported.

Method used

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  • Method for preparing diglyceride from crude fish oil of marine aquatic fishes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) the crude fish oil raw material of anchovy, mackerel and glycerin are mixed evenly, and the mol ratio of the substrate in this crude fish oil raw material and glycerin is 1:2.5;

[0026] (2) Add 0.5% immobilized lipase LipozymeRMIM to the material of step (1), and react at a temperature of 52° C. for 8 hours at a stirring rate of 150 r / min;

[0027] (3) After the reaction, the material in step (2) is filtered or centrifuged to remove the immobilized lipase LipozymeRMIM to obtain a mixture containing diglyceride;

[0028] (4) Molecular distillation or silica gel chromatography is carried out to the mixture obtained in step (3) to obtain a diglyceride product rich in n-3PUFA, and the diglyceride synthesis rate is 45%, wherein the crude fish oil in the present embodiment and the obtained The composition of fatty acids in diglyceride products is shown in the table below:

[0029]

Embodiment 2

[0031] (1) the crude fish oil raw material of anchovy, mackerel and glycerin are mixed evenly, and the mol ratio of the substrate in this crude fish oil raw material and glycerin is 1:2.5;

[0032] (2) Add 0.7% immobilized lipase LipozymeTLIM to the material of step (1), and react at a temperature of 50° C. for 8 hours at a stirring rate of 160 r / min;

[0033] (3) After the reaction, the material in step (2) is filtered or centrifuged to remove the immobilized lipase LipozymeTLIM to obtain a mixture containing diglyceride;

[0034] (4) Molecular distillation or silica gel chromatography is carried out to the mixture obtained in step (3) to obtain a diglyceride product rich in n-3PUFA, and the diglyceride synthesis rate is 44%, wherein the crude fish oil in the present embodiment and the obtained The comparison of the fatty acid composition in the diglyceride product is basically the same as in the examples.

Embodiment 3

[0036] (1) Mix the crude fish oil raw material of anchovy and mackerel with glycerin evenly, and the mol ratio of the substrate in this crude fish oil raw material and glycerin is 1:2;

[0037] (2) Add 0.6% immobilized lipase Novozym435 to the material in step (1), and react at a temperature of 40° C. for 6 hours at a stirring rate of 150 r / min;

[0038] (3) After the reaction, the material in step (2) is filtered or centrifuged to remove the immobilized lipase Novozym435 to obtain a mixture containing diglyceride;

[0039] (4) Molecular distillation or silica gel chromatography is carried out to the mixture obtained in step (3) to obtain a diglyceride product rich in n-3PUFA, and the diglyceride synthesis rate is 42%, wherein the crude fish oil in the present embodiment and the obtained The comparison of the fatty acid composition in the diglyceride product is basically the same as in the examples.

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Abstract

The invention discloses a method for preparing diglyceride from crude fish oil of marine aquatic fishes, wherein the method comprises the following steps: (1) uniformly mixing a crude fish oil raw material from marine aquatic fishes with glycerol; (2) adding 0.1-1.0% of immobilized lipase Lipozyme TMIM, Lipozyme TLIM or Novozym 435 to the materials of the step (1), stirring at rate of 150-170r / min and reacting at 40-80 DEG C for 6-10h; (3) after the reaction, filtering or centrifuging the materials of the step (2), and removing the immobilized lipase so as to obtain a mixture containing diglyceride; and (4) obtaining a finished diglyceride product from the mixture obtained from the step (3) through molecular distillation or silica gel chromatography. The method disclosed by the invention can achieve synthesis researches of the diglyceride by taking the crude fish oil prepared from leftovers of marine aquatic fishes as a raw material; and the method is capable of effectively improving the use value of aquatic leftovers, increasing the additional value of low-value fishes and reducing environmental pollution, and the method is conducive to the creation of economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of diglyceride production, and in particular relates to a method for preparing diglyceride from marine aquatic fish crude fish oil raw materials. Background technique [0002] Diacylglycerol is a natural component of oil and an intermediate product of oil metabolism in the human body. It is also a multifunctional additive with many advantages such as safety, nutrition, high human compatibility, and good processing adaptability. Therefore, it is used in food, It is widely used in medicine, chemical industry and other industries. [0003] Diglyceride edible oils currently on the market are mainly prepared from triglyceride (TAG) type oils such as rapeseed oil, soybean oil, palm oil, olive oil and peanut oil. Kahveci et al. used Novozyme435 to catalyze the synthesis of glycerin from rapeseed oil and glycerin Diester, diglyceride content reaches 40% to 50%. Valerio catalyzed the preparation of glycerin and oli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
Inventor 黄惠莉汪泳童记强张秀娟刘东阳张宝林
Owner HUAQIAO UNIVERSITY
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