A synthesis technology of decompressed azeotropic continuous dewatering esterase enzyme method

Abstract

The invention belongs to the technical field of biotechnology of non-water phase enzymatic organic synthesis application, and particularly relates to a synthesis technology of decompressed azeotropic continuous dewatering esterase enzyme method. The technology is suitable for the synthesis of each ester bond under the catalysis of biocatalyst (such as Novozym 435). The invention provides a universally applicable novel synthesis technology using esterase enzyme method, and can carry out continuous dewatering through decompressed azeotropy under the condition that the catalytic activity of the biocatalyst is not influenced, so as to lead the balanced reaction to move towards the synthetic direction of ester, thereby greatly increasing the conversion rate of enzymatic organic synthesis, ensuring the completion of the reaction; completely solving the problem of non-water phrase enzymatic organic synthesis, increasing purity and yield of the product and reducing manufacturing cost. The invention has wide application prospect in the fields of medicine, food, material, fine chemistry industry, etc.

Description

technical field [0001] The invention belongs to the field of applied biotechnology of non-aqueous phase enzymatic organic synthesis, in particular to an ester enzymatic synthesis process of vacuum azeotropic continuous water removal. Background technique [0002] Regarding the synthesis of esters, there are two types of methods: chemical synthesis and biocatalytic synthesis. The chemical synthesis process is very mature and is still widely used in the industrial production of organic esters. But there are some fatal flaws in the chemical synthesis process. This process often requires the use of strong liquid acids such as concentrated sulfuric acid or p-methoxybenzenesulfonic acid as a catalyst, and the reaction temperature is very high, usually accompanied by unavoidable side reactions, which have a great impact on the conversion rate of raw materials and the yield of products. Large, the separation and purification process is complicated, and it also has extremely seriou...

AI Technical Summary

Problems solved by technology

Molecular sieve adsorption method is convenient and easy to implement, with obvious effect, and is often used in laboratory research. However, since the regeneration of molecular sieve after adsorption saturation requires special equipment and technology, the investment cost is high, so it cannot be applied in large-scale industrial production.

Membrane separation and water absorption method is theoretically feasible, but it is still difficult to realize due to problems such as membrane ducer, aging and service life.

Use tert-amyl alcohol, tert-butanol, etc., which can be compatible with enzymes to ensure the catalytic activity of enzymes, and can form an azeotropic mixture with the generated water as the reaction medium and water agent, and use negative pressure azeotropic distillation It is a good choice to take the water away by means of water, but unfortunately, the formed azeotropic mixture does not separate after condensation, so the continuous separation of water and the continuous recycling of solvent cannot be realized. The distilled water-containing solvent must be After special drying treatment, it can be reused

Drying costs of aqueous solvents greatly limit the application of this technology