Stable black rice anthocyanin monomer and molecular modification preparation method

A technology of black rice flower and cyanin, which is applied in the field of pigment research, can solve the problems of limiting the application of microcapsules, etc., and achieve the effects of easy separation and purification, improved stability and high specificity

Active Publication Date: 2019-05-03
安徽一雄生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the application of natural active substances in the food industry is mainly improved through the microencapsulation method, but most of the microcaps

Method used

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  • Stable black rice anthocyanin monomer and molecular modification preparation method
  • Stable black rice anthocyanin monomer and molecular modification preparation method
  • Stable black rice anthocyanin monomer and molecular modification preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The preparation steps of cyanidin-3-glucoside (C3G) powder are as follows:

[0048] (1) Cleaning and removing impurities: removing impurities and moldy particles visible to the naked eye in black rice;

[0049](2) Grinding and extraction: crush the black rice after removal of impurities, and pass through a 50-mesh sieve; take black rice and water according to the mass-volume ratio of 1 Kg:10L, and then use 1% citric acid solution to adjust its pH value to 2.5 , at 20°C, leaching for 2 h to obtain black rice anthocyanin extract;

[0050] (3) Centrifugal separation: use a disc centrifuge at 5000 r / min to conduct solid-liquid separation of the black rice anthocyanin extract, remove starch, protein, polysaccharide and other impurities in the extract, and take the supernatant solution to obtain the black rice anthocyanin aqueous extract;

[0051] (4) Resin purification: use AB-8 resin to purify the water extract of black rice anthocyanins, first elute the column with 3 tim...

Embodiment 2

[0057] The preparation steps of cyanidin-3-glucoside (C3G) powder are as follows:

[0058] (1) Cleaning and removing impurities: remove the moldy particles that are visible to the naked eye in the black rice;

[0059] (2) Grinding and extracting: crush the black rice after removal of impurities, and pass through a 100-mesh sieve; take black rice and water according to the mass-volume ratio of 1:20 (Kg / L), and then use 3% citric acid solution to adjust the The pH is 6.0, at 55 ℃, leaching for 5 hours to obtain black rice anthocyanin extract;

[0060] (3) Centrifugal separation: use a disc centrifuge to separate the black rice anthocyanin extract from solid-liquid at 10,000 r / min, remove starch, protein, polysaccharide and other impurities in the extract, and take the above clear liquid to obtain black rice anthocyanin aqueous extract;

[0061] (4) Resin purification: use AB-8 resin to purify the aqueous extract of black rice anthocyanins, first elute the column with 9 times t...

Embodiment 3

[0067] The preparation steps of cyanidin-3-glucoside (C3G) powder are as follows:

[0068] (1) Cleaning and removing impurities: remove the moldy particles that are visible to the naked eye in the black rice;

[0069] (2) Grinding and extracting: crush the black rice after removal of impurities, and pass through a 100-mesh sieve; take black rice and water according to the mass-volume ratio of 1:20 (Kg / L), and then use 3% citric acid solution to adjust the The pH is 6.0, at 55 ℃, leaching for 5 hours to obtain black rice anthocyanin extract;

[0070] (3) Centrifugal separation: use a disc centrifuge to separate the black rice anthocyanin extract from solid-liquid at 10,000 r / min, remove starch, protein, polysaccharide and other impurities in the extract, and take the above clear liquid to obtain black rice anthocyanin aqueous extract;

[0071] (4) Resin purification: use AB-8 resin to purify the aqueous extract of black rice anthocyanins, first elute the column with 9 times t...

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Abstract

The invention relates to a molecular modification method for improving the stability of a black rice anthocyanin monomer (cyaniding-3-glucoside, C3G). The specific steps are as follows: (1) cleaning and impurity removing; (2) pulverization and extraction; (3) centrifugation; (4) resin purification; (5) nanofiltration concentration; (6) vacuum freeze-drying; (7) C3G extraction purification; (8) vacuum freeze-drying; (9) enzymatic modification of C3G; (10) separation of C3G modified product; and (11) vacuum freeze-drying. The method firstly uses the mild conditions to separate and purify the black rice anthocyanin monomer C3G from the black rice to ensure the integrity of a reaction substrate; and then performs the enzymatic modification of the C3G, the whole process is safe, efficient, thereaction condition is mild and easy to control, the selected Novozym 435 lipase has strong specificity and the modified product is single, s preparative liquid chromatography can be used for efficientseparating and purifying, and the modified product is dried by a vacuum freeze-drying technology to maximize the stability of the anthocyanin structure.

Description

technical field [0001] The invention belongs to the technical field of pigment research, and in particular relates to a black rice anthocyanin monomer (cyanidin-3-glucoside, C3G) with stable performance and a molecular modification preparation method. Background technique [0002] Anthocyanins, also known as anthocyanins, are water-soluble natural pigments that widely exist in plants in nature. They belong to flavonoid compounds and are also the main coloring substances in plant petals. In recent years, a lot of research has been done on the physiological functions of anthocyanins at home and abroad, and fruitful research results have been obtained. Studies have found that regular consumption of anthocyanins can significantly improve vision, relieve liver dysfunction, inhibit tumor activity, slow down the deposition of senile plaques, and have the function of anti-oxidation and anti-aging. At the same time, anthocyanin is still a relatively safe natural pigment. The develop...

Claims

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Application Information

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IPC IPC(8): C12P19/60C07H17/065C07H1/08
Inventor 孙汉巨朱勇生何述栋唐明明赵金龙涂李军张左勇孙先保余敏何钱王军辉操小栋叶永康
Owner 安徽一雄生物科技有限公司
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