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Amphipathic low-density lipoprotein adsorbent and preparation method thereof

A low-density lipoprotein and adsorbent technology, applied in chemical instruments and methods, other chemical processes, blood circulation treatment, etc., can solve the problems of high price, low selective adsorption capacity, etc., achieve high blood compatibility, improve The effect of selective scavenging capacity

Active Publication Date: 2015-11-11
佛山市博新生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In short, the selective adsorption capacity of the current LDL adsorbent is not high, and the adsorbent with high selective adsorption capacity is expensive, as high as more than 100,000 yuan

Method used

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  • Amphipathic low-density lipoprotein adsorbent and preparation method thereof

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preparation example Construction

[0037] The preparation method of amphipathic low-density lipoprotein adsorbent comprises the following steps:

[0038] 1) Epoxidizing the double bond or hydroxyl group on the carrier to obtain a modified carrier;

[0039] 2) Mixing and reacting the modified carrier with the taurine aqueous solution to immobilize the taurine;

[0040] 3) or mix the carrier loaded with taurine with the deoxycholic acid solution, add an amidation catalyst and adjust the pH, so that the deoxycholic acid is condensed and coupled to the taurine;

[0041] 4) After the reaction is complete, wash to obtain the amphipathic low-density lipoprotein adsorbent.

[0042] Further, the pH of the amidation reaction is 4-6.

[0043] Further, the catalysts for the amidation reaction are N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride.

[0044] Further, in the finally obtained adsorbent, the molar ratio of taurine to deoxycholic acid is (1-10):1, and the molar ratio of tauri...

Embodiment 1

[0051]1) Take polystyrene divinylbenzene resin and add 50mL of dichloroethane to soak overnight to make the resin swell, add 0.5% acetone solution of m-chloroperoxybenzoic acid, stir and react below 4°C to carry out epoxidation modification, and react After completion, wash with alcohol and purified water to obtain the adsorbent L0;

[0052] 2) Mix the adsorbent L0 with the taurine aqueous solution, shake and react at 60°C for 24 hours, wash with alcohol and purified water, and obtain the adsorbent L1;

[0053] 3) Mix 10g of adsorbent L1 with 20mL of 2.5g (12.5%) deoxycholic acid solution, adjust the pH to 4.8, add 0.8g of NHS (N-hydroxysuccinimide), and then slowly add 0.5g of 1-(3 -Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), reacted with shaking at 25°C for 4 hours, washed with alcohol and water for injection after the reaction was completed, and obtained adsorbent L2.

Embodiment 2

[0055] Sorbent synthesis

[0056] 2-1 Epoxy modification

[0057] Take a certain amount of polystyrene divinylbenzene resin and add 50mL of dichloroethane to soak overnight, add 20mL of 0.6% m-chloroperoxybenzoic acid in acetone solution dropwise, and stir in an ice-water bath (the temperature is controlled at 2°C) The reaction was carried out for 24 hours, and the rotation speed was 200 rpm. After the reaction is completed, wash with alcohol and purified water.

[0058] 2-2 Immobilized Taurine

[0059] Take 2-1 resin, add 1.2g of taurine aqueous solution 50mL, shake and react at 60°C for 24 hours. After the reaction is completed, wash with alcohol and purified water. get the adsorbent.

[0060] 2-3 condensed deoxycholic acid

[0061] Take the adsorbent obtained in 2-2, add 1g or 4g of deoxycholic acid solution 20mL, adjust the pH to 4.8, add 0.8g of NHS (N-hydroxysuccinimide), and then slowly add 0.5g of 1-(3-dimethyl Aminopropyl)-3-ethylcarbodiimide hydrochloride (EDC...

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Abstract

The invention discloses an amphipathic low-density lipoprotein adsorbent and a preparation method thereof. A skeleton of the adsorbent is a carrier, taurine is immobilized on the surface of the carrier, and deoxycholic acid is coupled on the taurine. According to the amphipathic low-density lipoprotein adsorbent disclosed by the invention, by using electrostatic interaction of a sulfo group, LDL (low-density lipoprotein) can be effectively adsorbed and removed; condensed deoxycholic acid is further utilized, adsorption to beneficial element HDL (high density lipoprotein) is reduced through the hydrophobic and steric hindrance effects of the naphthene structure, total cholesterol (TC) and triglyceride(TG) can be removed, and the selective removal ability of the adsorbent is improved. The amphipathic low-density lipoprotein adsorbent disclosed by the invention carries the hydrophilic sulfonic group and hydrophobic deoxycholic acid, can simulate hydrophilic and hydrophobic structures of phospholipid molecules, has better blood compatibility with cells, and is suitable for whole blood perfusion. By adjusting the ratio of the hydrophilic and hydrophobic groups, the adsorbent with high blood compatibility and capable of specifically adsorbing LDL (low-density lipoprotein) can be obtained.

Description

technical field [0001] The invention relates to a medical adsorption material for blood perfusion, in particular to a low-density lipoprotein adsorbent and a preparation method thereof. Background technique [0002] Excessive low-density lipoprotein (LDL) concentration is the most common and unhealthy condition, which is associated with rapid proliferation of atherosclerotic plaque, arteriosclerosis, and premature severe cardiovascular disease, even death. Cardiovascular disease is one of the three major diseases that threaten human health. About 8 to 10 million people die from cardiovascular disease and stroke caused by arteriosclerosis every year in the world, while about 4 million people die from cardiovascular and cerebrovascular diseases in my country every year. . [0003] The content ratio of low-density and high-density lipoprotein (HDL) in serum is 1:2. Both have important tasks: low-density lipoprotein transports cholesterol from the liver to body tissues, and hig...

Claims

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Application Information

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IPC IPC(8): B01J20/22B01J20/26B01J20/30A61M1/36
Inventor 李设桥姜建明
Owner 佛山市博新生物科技有限公司
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