Microbial complex flora for eliminating sulfide in sediments

A compound flora and sulfide technology, applied in the methods of using microorganisms, microorganism-based methods, microorganisms, etc., can solve the problems of elimination, narrow ecological niche of pure bacteria, low biomass, etc., and achieve the maintenance of structure and composition. Reduce the effect of the adaptation process

Active Publication Date: 2015-11-11
OCEAN UNIV OF CHINA
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Problems solved by technology

[0004] The purpose of the present invention is to provide a microbial complex flora for eliminating sulfide in sediments, which can solve the problem of narrow ecological niche of pure bacteria, inability to adapt to sediment environment, easy elimination in a relatively short period of time, low biomass, and low metabolic rate. Slow etc.

Method used

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  • Microbial complex flora for eliminating sulfide in sediments
  • Microbial complex flora for eliminating sulfide in sediments
  • Microbial complex flora for eliminating sulfide in sediments

Examples

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Embodiment 1

[0025] Example 1: Screening, identification and genetic stability of microbial complexes for sulfide elimination in sediments

[0026] (1) Screening of microbial complex flora for sulfide elimination in sediments

[0027] 1) Collect the surface sediments within 10cm of depth in offshore aquaculture areas, and inoculate at a volume percentage of 10% to make a bacterial suspension:

[0028] In an Erlenmeyer flask, inoculate sterilized old seawater with a volume percentage of 10% of the collected surface sediment within a depth of 10 cm in an offshore culture area. Seal the Erlenmeyer flask with aluminum foil and place it on a constant temperature shaker at 30°C and shake at 120r / min for 30min to fully release the bacteria in the sediment. After shaking, let it stand for 30 minutes until the sediment re-precipitates, and the supernatant is the bacterial suspension;

[0029] 2) Inoculate the bacterial suspension in the above step 1) with an inoculum size of 10% by volume percent...

Embodiment 2

[0053] Embodiment 2: This study microbial compound bacterial agent is to the removal effect of sulfide

[0054] 1) Quickly thaw the microbial complex flora stored at -80°C in step 6) of the screening of microbial complex flora for sulfide elimination in sediments determined in Example 1 in a 30°C water bath;

[0055] 2) Inoculate the microbial complex flora after thawing in step 1) in an inoculum size of 10% by volume percentage in an inorganic salt medium with an initial concentration of sulfide of 1000 mg / l, in an anaerobic bottle at 30° C. , 150r / min constant temperature shaking culture. Among them, the inorganic salt medium is: K 2 HPO 4 : 1.2g / l, KH 2 PO 4 : 1.2g / l, NH 4 Cl: 0.4g / l, MgCl 2 : 0.2g / l, ferric citrate: 0.01g / l, NaHCO 3 : 2g / l, Na 2 S 2 o 3 ·5H 2 O: 3.875g / l, prepared with sterilized aged sea water.

[0056] At the same time, the microorganisms identified in Example 1 were selected for specific strains (Vibrio mimeticus, Desulfovibrio, Pseudomonas ...

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Abstract

The invention aims to provide a microbial complex flora for eliminating sulfide in sediments. By the aid of the microbial complex flora, problems of narrow ecological niche, incapability of adapting to sediment environments, easiness in being sifting out within a short time, low biomass, slow metabolism and the like of pure bacteria can be solved. The microbial complex flora contains bacillus (Bacillus spp.), vibrio (Vibrio spp.), pseudomonas (Pseudomonas spp.) and desulfovibrio (Desulfovibrio spp.). The microbial complex flora has the advantages that bacteria of the microbial complex flora come from the sediments in inshore culture zones with high sulfide contents and are acquired by means of domestication, and accordingly adaptation procedures due to severe environment difference can be omitted during application; the excellent stability of the structure of the flora can be kept in sulfide removing procedures.

Description

technical field [0001] The invention belongs to the technical field of functional microbial complex flora screening, and in particular relates to a microbial complex flora, flora composition, flora genetic stability and a preparation method thereof for the treatment of sulfide in mariculture sediments. technical background [0002] my country is a big country in mariculture. In offshore aquaculture areas, in order to increase the output of mariculture, people often add a large amount of organic bait to the aquaculture area, resulting in serious organic pollution of the water body in the aquaculture area. In the sediment-water interface environment in offshore aquaculture areas, the aerobic degradation of organic matter leads to a large consumption of dissolved oxygen in the water body, resulting in a decrease in the oxidation-reduction potential (Eh) of the water body. Under these conditions, the sulfate-reducing bacteria (SRB) in the sediment will produce a large amount of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20C02F11/02C12R1/07C12R1/63C12R1/38C12R1/01
Inventor 赵阳国郑宇白洁田伟君胡泓佘宗莲高孟春郭亮
Owner OCEAN UNIV OF CHINA
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